Method development of Amlodipine and Nevirapine by UVS and HPTLC - Couverture souple

Dinakaran, Sathis Kumar; Yandamuri, Narayudu

 
9783659630613: Method development of Amlodipine and Nevirapine by UVS and HPTLC
Couverture souple
ISBN 10 : 3659630616 ISBN 13 : 9783659630613
Editeur : LAP Lambert Academic Publishing, 2014

Synopsis

A simple, economical, and accurate absorption ratio method in UV Spectroscopy and HPTLC method were developed for the simultaneous estimation of Amlodipine besylate (AML) and Nevirapine (NEV) in bulk and tablet dosage form. In Spectroscopy, 0.1M HCl was used as a diluent to dissolve AML and NEV. The absorptions were observed at 252 nm (isobestic point) and 295 nm (λmax of NEV), which were selected based on overlapping spectra of AML and NEV. The linearity range of the UV method was found to be 3-7 µg/ml at 252 nm for AML (r2=0.9995±0.0005) and 295 nm for NEV (r2=0.9985±0.0012). HPTLC method was developed with silica gel GF TLC Plate. Chloroform-Methanol-Glacial acetic acid (75.8:21.6:2.6) was used as a diluent. The spot after the chromatogram was identified in UV and Iodine chamber. The linearity range of HPTLC method was found to be 5-25 µg/ml for AML (r2=0.9994) and 295 nm for NEV (r2=0.999). Recovery study of UV and HPTLC methods were performed to confirm the accuracy of the methods. The methods were validated as per ICH guidelines. The methods were found to be simple, precise, accurate and rapid for the simultaneous determination of AML and NEV in bulk and tablet dosage form.

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À propos de l'auteur

D.Sathis Kumar is working as an associate professor in Aditya Institute of Pharmaceutical Sciences and Research, AP, India. He have more than 90 publication in reputed journals. He has secured honors/awards from IBC, Cambridge, England. His biography was included in Marquis Who’s Who in the world.

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Éditeur
LAP Lambert Academic Publishing
Date d'édition
2014
Langue
anglais
ISBN 10 
3659630616
ISBN 13 
9783659630613
Reliure
Broché
Nombre de pages
92
Coordonnées du fabricant
non disponible
Personne responsable
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