Le coton est une culture importante. Au cours des dernières années, la maladie de l'enroulement des feuilles de coton (CLCuD) est devenue endémique. Il a fourni un énorme obstacle à la production de rendements élevés en coton. Cette maladie est causée par un groupe de begomovirus, à savoir la maladie de la boucle des feuilles de coton (CLCuD). Plusieurs approches d'élevage et transgéniques ont été essayées pour surmonter le problème. Parmi ces interférences ARN (ARN) s'est avérée plus prometteuse. L'utilisation de l'interférence ARN est favorable car la technique est respectueuse de l'environnement. Dans les travaux de recherche actuels, la construction génique basée sur l'ARNi a été transformée en deux versions de coton d'élite MNH-786 et VH-289 pour améliorer la résistance virale contre CLCuD. La transformation de la construction dans les plantes a été effectuée par la méthode Agrobacterium médiated shoot apex. La transformation et l'intégration réussies de la construction d'ARNi à base d'amplicon ont été confirmées par PCR dans la génération T1 et T2 en utilisant des amorces spécifiques aux gènes. La capacité d'abaissement de la construction C2 a été confirmée par la PCR du titre CLCuD en temps réel. La comparaison de l'indice de maladie virale et du titre viral a assuré que les plantes présentant une intégration positive du gène de l'ARNi avec des symptômes minimaux avaient également un faible titre viral.
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
Vendeur : BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Allemagne
Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Cotton is an important crop. During past few years, cotton leaf curl disease (CLCuD) has emerged as an endemic. It has provided huge hindrance in production of high cotton yields. This disease is caused by cluster of begomovirus namely Cotton Leaf Curl Disease (CLCuD). Several breeding and transgenic approaches have been tried to overcome the problem. Among these RNA interference (RNAi) proved to be more promising. Use of RNA interference is favorable as the technique is environment friendly. In current research work RNAi based gene construct was transformed in two elite cotton verities MNH-786 and VH-289 to enhance viral resistance against CLCuD. The transformation of construct in plants was done by Agrobacterium mediated shoot apex method. The successful transformation and integration of the amplicon based RNAi construct was confirmed through PCR in T1 and T2 generation by using gene specific primers. Knock down ability of C2 construct was confirmed through real time CLCuD titer PCR. Comparison of virus disease index and Virus titer assured that the plants showing positive RNAi gene integration with minimum symptoms also had low viral titer. 184 pp. Englisch. N° de réf. du vendeur 9783659698514
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Vendeur : moluna, Greven, Allemagne
Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Yasmeen AneelaAneela Yasmeen M. Phil: Molecular Biology RO cum Lecturer: Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore. Executive Member: National Academy of Young Scientists (NAYS), Pakistan Foun. N° de réf. du vendeur 158961856
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Vendeur : AHA-BUCH GmbH, Einbeck, Allemagne
Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Cotton is an important crop. During past few years, cotton leaf curl disease (CLCuD) has emerged as an endemic. It has provided huge hindrance in production of high cotton yields. This disease is caused by cluster of begomovirus namely Cotton Leaf Curl Disease (CLCuD). Several breeding and transgenic approaches have been tried to overcome the problem. Among these RNA interference (RNAi) proved to be more promising. Use of RNA interference is favorable as the technique is environment friendly. In current research work RNAi based gene construct was transformed in two elite cotton verities MNH-786 and VH-289 to enhance viral resistance against CLCuD. The transformation of construct in plants was done by Agrobacterium mediated shoot apex method. The successful transformation and integration of the amplicon based RNAi construct was confirmed through PCR in T1 and T2 generation by using gene specific primers. Knock down ability of C2 construct was confirmed through real time CLCuD titer PCR. Comparison of virus disease index and Virus titer assured that the plants showing positive RNAi gene integration with minimum symptoms also had low viral titer. N° de réf. du vendeur 9783659698514
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Vendeur : preigu, Osnabrück, Allemagne
Taschenbuch. Etat : Neu. Transformation of Cotton with siRNA based Construct Targeting CLCuD | Transformation of Cotton (Gossypium hirsutum) with Amplicon Based Small Interfering RNA Targeting CLCuD | Aneela Yasmeen (u. a.) | Taschenbuch | 184 S. | Englisch | 2015 | LAP LAMBERT Academic Publishing | EAN 9783659698514 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. N° de réf. du vendeur 104678487
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Vendeur : buchversandmimpf2000, Emtmannsberg, BAYE, Allemagne
Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -Cotton is an important crop. During past few years, cotton leaf curl disease (CLCuD) has emerged as an endemic. It has provided huge hindrance in production of high cotton yields. This disease is caused by cluster of begomovirus namely Cotton Leaf Curl Disease (CLCuD). Several breeding and transgenic approaches have been tried to overcome the problem. Among these RNA interference (RNAi) proved to be more promising. Use of RNA interference is favorable as the technique is environment friendly. In current research work RNAi based gene construct was transformed in two elite cotton verities MNH-786 and VH-289 to enhance viral resistance against CLCuD. The transformation of construct in plants was done by Agrobacterium mediated shoot apex method. The successful transformation and integration of the amplicon based RNAi construct was confirmed through PCR in T1 and T2 generation by using gene specific primers. Knock down ability of C2 construct was confirmed through real time CLCuD titer PCR. Comparison of virus disease index and Virus titer assured that the plants showing positive RNAi gene integration with minimum symptoms also had low viral titer.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 184 pp. Englisch. N° de réf. du vendeur 9783659698514
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Vendeur : Mispah books, Redhill, SURRE, Royaume-Uni
paperback. Etat : Like New. LIKE NEW. SHIPS FROM MULTIPLE LOCATIONS. book. N° de réf. du vendeur ERICA80036596985126
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