Recombinant Protein Production: A Case Study Research Report - Couverture souple

Patrício, Catarina; Antunes, Sandra; Domingos, Ana

 
9783659781704: Recombinant Protein Production: A Case Study Research Report
Couverture souple
ISBN 10 : 3659781703 ISBN 13 : 9783659781704
Editeur : LAP LAMBERT Academic Publishing, 2015

Synopsis

Plasmodium spp. and Leishmania are the causing parasites to malaria and leishmaniasis, two widely spread diseases that are still responsible for millions of cases worldwide every year. The proteins in study, AGAP007752-PA (Anopheles gambiae) and α-tubulin (Plasmodium spp. and Leishmania infantum), are thought to be relevant in decreasing parasite transmission by affecting their life cycles. The aim is to use recombinant versions of those proteins to develop new control strategies to diminish disease incidence. In order to further the AGAP007752-PA research, the gene was amplified, cloned and sequenced. After, all three proteins were expressed in E. coli, and the recombinant proteins analysed by SDS-PAGE and Western Blot, again verifying their identities by sequencing. After the procedures’ optimization, AGAP007752 gene amplification was achieved, although this protein was not successfully expressed. Contrarily, alfa-tubulins had been previously cloned, and the proteins’ expression was now achieved. The research can be furthered, either through the production of the recombinant protein (AGAP007752-PA) or through purification of the expressed recombinant α-tubulin proteins.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Plasmodium spp. and Leishmania are the causing parasites to malaria and leishmaniasis, two widely spread diseases that are still responsible for millions of cases worldwide every year. The proteins in study, AGAP007752-PA (Anopheles gambiae) and α-tubulin (Plasmodium spp. and Leishmania infantum), are thought to be relevant in decreasing parasite transmission by affecting their life cycles. The aim is to use recombinant versions of those proteins to develop new control strategies to diminish disease incidence. In order to further the AGAP007752-PA research, the gene was amplified, cloned and sequenced. After, all three proteins were expressed in E. coli, and the recombinant proteins analysed by SDS-PAGE and Western Blot, again verifying their identities by sequencing. After the procedures’ optimization, AGAP007752 gene amplification was achieved, although this protein was not successfully expressed. Contrarily, alfa-tubulins had been previously cloned, and the proteins’ expression was now achieved. The research can be furthered, either through the production of the recombinant protein (AGAP007752-PA) or through purification of the expressed recombinant α-tubulin proteins.

Biographie de l'auteur

Catarina Patrício was born in 1991. In 2009 she started an undergratuate degree in Pharmacy, concluding it in 2015. This book is based on the research carried out for the said degree.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2015
Langue
anglais
ISBN 10 
3659781703
ISBN 13 
9783659781704
Reliure
Broché
Nombre de pages
88
Coordonnées du fabricant
non disponible
Personne responsable
KreGa Onlinehandel GmbH
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