Identification of Molecular Marker for Introgression of MYMV: Mungbean yellow mosaic virus - Couverture souple

Kalaria, Rishee; Mahatma, Lalit

 
9783659788062: Identification of Molecular Marker for Introgression of MYMV: Mungbean yellow mosaic virus
Couverture souple
ISBN 10 : 3659788066 ISBN 13 : 9783659788062
Editeur : LAP LAMBERT Academic Publishing, 2016

Synopsis

Twenty six germplasm lines were screened at the hot spot of Pulse Research Station (PRS), Navsari for the finding resistant source of MYMV. Four germplasm lines viz., NAUMR1, NAUMR2, NAUMR3 and MEHA showed highly resistant reaction against YMD. NAUMR1, NAUMR2 and NAUMR3 were found late maturing, tall and produced few pods only. MEHA is MYMV resistant mungbean cultivar developed and released from IIPR, Kanpur in 2004, however, is of small size. Out of 200 RAPD markers, only fourteen markers showed variable degree of polymorphism. Maximum 90% polymorphism was observed in OPG-5, OPJ-18 and OPM-20 whereas minimum polymorphism (28.57 per cent) was in OPH-9. Overall polymorphism was found to be 70.17 per cent. Fourteen markers on five genotypes generated 126 bands out of which 90 were polymorphic band. OPG-5, OPJ-18 and OPM-20 were proved to be the best markers for the study of polymorphism as it produced 28, 35, 28 amplicons respectively. In OPG-5 a band of size ~850bp was absent in susceptible cultivar GM4 while present in all the resistant lines. In the marker OPJ-18, total 6 unique band of approximately 250bp, 300bp, 450bp, 500bp, 850bp and 1250bp size were present in all resistant.

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Présentation de l'éditeur

Twenty six germplasm lines were screened at the hot spot of Pulse Research Station (PRS), Navsari for the finding resistant source of MYMV. Four germplasm lines viz., NAUMR1, NAUMR2, NAUMR3 and MEHA showed highly resistant reaction against YMD. NAUMR1, NAUMR2 and NAUMR3 were found late maturing, tall and produced few pods only. MEHA is MYMV resistant mungbean cultivar developed and released from IIPR, Kanpur in 2004, however, is of small size. Out of 200 RAPD markers, only fourteen markers showed variable degree of polymorphism. Maximum 90% polymorphism was observed in OPG-5, OPJ-18 and OPM-20 whereas minimum polymorphism (28.57 per cent) was in OPH-9. Overall polymorphism was found to be 70.17 per cent. Fourteen markers on five genotypes generated 126 bands out of which 90 were polymorphic band. OPG-5, OPJ-18 and OPM-20 were proved to be the best markers for the study of polymorphism as it produced 28, 35, 28 amplicons respectively. In OPG-5 a band of size ~850bp was absent in susceptible cultivar GM4 while present in all the resistant lines. In the marker OPJ-18, total 6 unique band of approximately 250bp, 300bp, 450bp, 500bp, 850bp and 1250bp size were present in all resistant.

Biographie de l'auteur

Dr. R. K. Kalaria, Assistant Prof at Dept of Animal Biotechnology, Aspee Shakilam Agri Biotechnology Institute, Navsari Agricultural University, Ghod Doad Road, Athwa Farm, Surat (Gujarat), India. He is mainly engage in Teaching, Research and Extension. His main areas of research are Molecular virology, Molecular marker and biotic & abiotic stress.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2016
Langue
anglais
ISBN 10 
3659788066
ISBN 13 
9783659788062
Reliure
Broché
Nombre de pages
164
Coordonnées du fabricant
non disponible
Personne responsable
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