ZebraTrack: An Automated Method to Study Zebrafish Behaviour - Couverture souple

Bhargava, Yogesh; Bhargava, Anamika

 
9783659900518: ZebraTrack: An Automated Method to Study Zebrafish Behaviour
Couverture souple
ISBN 10 : 3659900516 ISBN 13 : 9783659900518
Editeur : LAP LAMBERT Academic Publishing, 2017

Synopsis

Behavioural neuroscience relies on software driven methods for behavioural assessment. Therefore, we propose a novel method which we called as ZebraTrack. It includes cost-effective imaging setup for distraction-free behavioural acquisition, automated tracking using open-source ImageJ software and workflow for extraction of behavioural endpoints. Our ImageJ algorithm is capable of providing control to users at key steps while maintaining automation in tracking without the need for the installation of external plugins. We have validated this method by testing novelty induced anxiety behaviour in adult zebrafish. Our results, in agreement with established findings, showed that during state-anxiety, zebrafish showed reduced distance travelled, increased thigmotaxis and freezing events. Furthermore, we proposed a method to represent both spatial and temporal distribution of choice-based behaviour which is currently not possible to represent using simple videograms. ZebraTrack method is simple and economical, yet robust enough to give results comparable with those obtained from costly proprietary software like Ethovision XT.

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Présentation de l'éditeur

Behavioural neuroscience relies on software driven methods for behavioural assessment. Therefore, we propose a novel method which we called as ZebraTrack. It includes cost-effective imaging setup for distraction-free behavioural acquisition, automated tracking using open-source ImageJ software and workflow for extraction of behavioural endpoints. Our ImageJ algorithm is capable of providing control to users at key steps while maintaining automation in tracking without the need for the installation of external plugins. We have validated this method by testing novelty induced anxiety behaviour in adult zebrafish. Our results, in agreement with established findings, showed that during state-anxiety, zebrafish showed reduced distance travelled, increased thigmotaxis and freezing events. Furthermore, we proposed a method to represent both spatial and temporal distribution of choice-based behaviour which is currently not possible to represent using simple videograms. ZebraTrack method is simple and economical, yet robust enough to give results comparable with those obtained from costly proprietary software like Ethovision XT.

Biographie de l'auteur

Dr. Yogesh Bhargava, PhD: Assistant Professor at Dr. Harisingh Gour Central University, Sagar, MP, India. Expert in Zebrafish Behavioural Neuroscience and interested in Technology development. Dr. Anamika Bhargava, PhD: Assistant Professor at Indian Institute of Technology, Hyderabad, India. Expert in Molecular Biology and Electrophysiology.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2017
Langue
anglais
ISBN 10 
3659900516
ISBN 13 
9783659900518
Reliure
Broché
Nombre de pages
60
Coordonnées du fabricant
non disponible
Personne responsable
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