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Molecular Characterization of Some Viruses Infecting Potatoes: In North Egypt Using Advanced Techniques - Couverture souple

Aseel, Dalia

 
9783659909924: Molecular Characterization of Some Viruses Infecting Potatoes: In North Egypt Using Advanced Techniques
Couverture souple
ISBN 10 : 3659909920 ISBN 13 : 9783659909924
Editeur : LAP LAMBERT Academic Publishing, 2017

Synopsis

Potato (Solanum tuberosum L.) is considered as one of the most economically important solanaceous crops that are cultivated in different regions in Egypt. In contrast, potato is a host to many bacterial; fungal and viral diseases. More than three dozen plant viruses; a viroid and phytoplasmas can infect potato crop and hardly reduce the yields.The present study aims to detect and characterize potato leaf roll virus (PLRV) and potato virus Y (PVY). To achieve this purpose, isolates of the viruses were randomly collected from infected leaves of potato from different regions of 7 different Egyptian Localities (EL-Beheira; EL-Gharbia; Alexandria; EL-Dakahlya; Elkalyobia; EL-Menofia and Matrouh), and some biological; serological and molecular techniques were carried out. In addition, some viral genes were identified and PLRV coat protein (CP) gene was expressed. The expressed proteins were purified to use it as an antigen and induce its polyclonal antiserum in rabbit blood cells for the detection of PLRV in infected plants. Moreover, the defense system response was detected and quantify for infected potato plant with PLRV or/and PVY.

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Présentation de l'éditeur

Potato (Solanum tuberosum L.) is considered as one of the most economically important solanaceous crops that are cultivated in different regions in Egypt. In contrast, potato is a host to many bacterial; fungal and viral diseases. More than three dozen plant viruses; a viroid and phytoplasmas can infect potato crop and hardly reduce the yields.The present study aims to detect and characterize potato leaf roll virus (PLRV) and potato virus Y (PVY). To achieve this purpose, isolates of the viruses were randomly collected from infected leaves of potato from different regions of 7 different Egyptian Localities (EL-Beheira; EL-Gharbia; Alexandria; EL-Dakahlya; Elkalyobia; EL-Menofia and Matrouh), and some biological; serological and molecular techniques were carried out. In addition, some viral genes were identified and PLRV coat protein (CP) gene was expressed. The expressed proteins were purified to use it as an antigen and induce its polyclonal antiserum in rabbit blood cells for the detection of PLRV in infected plants. Moreover, the defense system response was detected and quantify for infected potato plant with PLRV or/and PVY.

Biographie de l'auteur

Dr. Dalia Gamil Aseel Ahmed MohamedResearcher, Plant Protection and Bio molecular Diagnosis Department, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SARTA, City), Alexandria, Egypt. PhD in 2015, Faculty of Agriculture, Alexandria University.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2017
Langue
anglais
ISBN 10 
3659909920
ISBN 13 
9783659909924
Reliure
Broché
Nombre de pages
172
Coordonnées du fabricant
non disponible
Personne responsable
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