Isolation and Molecular Detection of Bacterial AHCs Degradation genes: Microbiological and Molecular Study - Couverture souple

Nasier, Mahmoud

 
9783659918513: Isolation and Molecular Detection of Bacterial AHCs Degradation genes: Microbiological and Molecular Study
Couverture souple
ISBN 10 : 3659918512 ISBN 13 : 9783659918513
Editeur : LAP LAMBERT Academic Publishing, 2016

Synopsis

Sixty three different aerobic bacterial colonies were isolated from sewage and sludge samples as degraders of aromatic hydrocarbons. Four isolates were selected as the most potent isolates capable of growing on mineral media supplemented with BTXHB components. These four isolates were selected for identification and H12 was chosen for further investigation in this study. Morphological and biochemical characterization was achieved for the four isolates. Amplification of 16S rDNA gene was used for identification of isolated strains. Some culturing conditions that affect the growth and the biodegradation capability of the bacterial isolate H12 were explored. Pseudomonas sp. H12 was examined on mineral media amended by BTXHB components, individually and in a mixture, each as a sole carbon source in 5 different concentrations and different removal efficiencies were recorded. Plasmid mini-prep and plasmid curing experiments demonstrated that biodegradation genes of Pseudomonas sp. H12 are extra-chromosomal. PCR amplification of the BTXHB catabolic genes demonstrated that the experimental bacterium carries the genes tmoA, xylM, xylA, xylE1.

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À propos de l'auteur

Mahmoud M. Nasier was born in Egypt 1981. He studied at Alazhar University where he successfully completed his degree of B.Sc. in Microbiology 2003 with excellence. As a scholar he continued for the master at the Genetic Engineering and Biotechnology Research Institute and Alexandria University for M.Sc. in Microbiology which was achieved in 2008.

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Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2016
Langue
anglais
ISBN 10 
3659918512
ISBN 13 
9783659918513
Reliure
Broché
Nombre de pages
88
Coordonnées du fabricant
non disponible
Personne responsable
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