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Development of bacterial biofilms using E.coli and P.aeruginosa - Couverture souple

Enitinwa, Tolulope

 
9783659921025: Development of bacterial biofilms using E.coli and P.aeruginosa
Couverture souple
ISBN 10 : 3659921025 ISBN 13 : 9783659921025
Editeur : LAP LAMBERT Academic Publishing, 2016

Synopsis

Biofilm bacteria existence in both natural and artificial surfaces is becoming increasingly evident. Biofilms are complex group of microorganisms, able to adapt and survive in a new environment provided there is an availability of nutrient, adequate incubation time and an appropriate media. The bacterial strains of Escherichia coli and Pseudomonas aeruginosa were used to develop biofilms in the laboratory. These bacterial strains were observed using Gram stain, Catalase test and Oxidase test. These bacterial strains were enumerated for viable colony units at 0, 2, 4, 6 and 8 hours and incubated at 30°C and 37°C respectively. Bacterial strains grown to stationary phase were inoculated into 96-well microtitre plate at 0, 2, 4, 6 and 8 hours incubated at 30°C and 37°C respectively, stained with crystal violet, followed by optical density measurement at 540nm. Result showed that E. coli and P. aeruginosa are Gram-negative facultative anaerobes, biofilms and viable CPU were obtained from both bacterial strains. The absorbance of adherent cells of the bacterial strains of E. coli and P. aeruginosa increased as the time increased from 0 to 6 hours and decreased at 8 hours respectively.

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Présentation de l'éditeur

Biofilm bacteria existence in both natural and artificial surfaces is becoming increasingly evident. Biofilms are complex group of microorganisms, able to adapt and survive in a new environment provided there is an availability of nutrient, adequate incubation time and an appropriate media. The bacterial strains of Escherichia coli and Pseudomonas aeruginosa were used to develop biofilms in the laboratory. These bacterial strains were observed using Gram stain, Catalase test and Oxidase test. These bacterial strains were enumerated for viable colony units at 0, 2, 4, 6 and 8 hours and incubated at 30°C and 37°C respectively. Bacterial strains grown to stationary phase were inoculated into 96-well microtitre plate at 0, 2, 4, 6 and 8 hours incubated at 30°C and 37°C respectively, stained with crystal violet, followed by optical density measurement at 540nm. Result showed that E. coli and P. aeruginosa are Gram-negative facultative anaerobes, biofilms and viable CPU were obtained from both bacterial strains. The absorbance of adherent cells of the bacterial strains of E. coli and P. aeruginosa increased as the time increased from 0 to 6 hours and decreased at 8 hours respectively.

Biographie de l'auteur

Tolulope Enitinwa; MSc: Studied Biotechnology at the University of Hertfordshire. Currently studying MSc. Project Management at the University of Hertfordshire, United Kingdom.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2016
Langue
anglais
ISBN 10 
3659921025
ISBN 13 
9783659921025
Reliure
Broché
Nombre de pages
60
Coordonnées du fabricant
non disponible
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