Puumala and Dobrava Hantaviruses: Development of PCR Protocols - Couverture souple

Smajlović Skenderagić, Lejla; Hukić, Mirsada

Couverture souple

ISBN 10 : 3659926531 ISBN 13 : 9783659926532

Editeur : LAP LAMBERT Academic Publishing, 2021

Synopsis

Throughout the world, nearly 150.000 persons are infected with hantaviruses each year. Hantaviruses are divided into two groups based on their human pathogenicity: Old World hantaviruses causing hemorrhagic fever with renal syndrome (HFRS) and New World hantaviruses causing hantavirus pulmonary syndrome (HPS). HFRS is an acute disease characterised by vascular bleeding and kidney damage, and, in most cases, it is not lethal. Mild forms of HFRS are usually caused by Puumala virus (PUUV), while the most severe forms of HFRS in Europe are caused by Dobrava-Belgrade virus (DOBV). Therefore, sensitive and specific molecular detection is essential for the timely diagnosis of hantavirus infections. In a typical laboratory context, we present the creation of nested, degenerate primers for detection of S and M segment of DOBV and M segment of PUUV, optimisation of various PCR components and cycling parameters, using both external and internal primers. As a result, this book provides a resource for anyone interested in conducting similar experiments and procedures.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2021
• Langue: anglais
• ISBN 10: 3659926531
• ISBN 13: 9783659926532
• Reliure: Broché
• Nombre de pages: 84
• Coordonnées du fabricant: non disponible
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