Articles liés à A Laboratory Guide to Biotin-Labeling in Biomolecule...
Synopsis
2.1 Introduction ...32 2.2 Technical Procedures ...32 Chemical Synthesis ...35 2.3 Results and Discussion...38 ...Chemical Synthesis ...38 Binding to anti-CCK antisera and CCK-B receptors ...40 Binding to streptavidin agarose and photoelution ...40 Affinity chromatography ...41 2.4 Troubleshooting...43 References ...44 ...Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ...45 Summary ...45 3.1 Introduction ...46 ...3.2 Technical Procedures ...48 Preparation of a Biotinylated Ligand ...48 Preparation of Membrane Fraction ...50 Solubilization of the PACAP Receptor ...51 Partial Purification of the PACAP Receptor ...52 Affinity Purification of the PACAP Receptor ...54 Final Purification of the PACAP Receptor ...56 Receptor-Binding Assay ...57 3.3 Results and Discussion...58 ...3.4 Troubleshooting ...61 Impurity in PACAP27-Cys-NH...61 2 Low specific activity of the PACAP receptor despite a single band ...62 Acknowledgments ...62 References ...63 ...Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ..." 65 Summary ...65 4.1 Introduction...66 . 4.2 Technical Procedures ..." 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ...67 Synthesis of photo reactive biotinylated peptide hormones ...67 Site-specific incorporation of biotin and photo labels in separate steps ...69 Photoaffinity labeling ...72 4.3 Results and Discussion ...76 Synthesis of photoactivatable insulins with permanent biotin labels ...76 Applications: Insulin ...78 VI Examples for other applications ...78 4.4 Troubleshooting...79 References ...81 ...
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Birkhauser, Basel, 1996
ISBN 10 : 376435206X
ISBN 13 : 9783764352066
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Hardcover. Etat : ex library-good. BioMethods Vol. 7. xii, [2], 237, [3] p. 24 cm. Ex library with labels on lower spine and rear pastedown, ink stamps on top edge, title and rear page. N° de réf. du vendeur 142372
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
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Birkhauser, 1996
ISBN 10 : 376435206X
ISBN 13 : 9783764352066
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A Laboratory Guide to Biotin-labelling in Biomolecule Analysis (Biomethods)
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Birkhäuser Basel, 2002
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ISBN 13 : 9783764352066
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
Edité par
Birkhäuser, 2002
ISBN 10 : 376435206X
ISBN 13 : 9783764352066
Ancien ou d'occasion
Couverture rigide
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