Stem cell-based therapy of ocular surface diseases: Novel approaches to expansion of adult epithelial stem cells in culture - Couverture souple

Meyer-Blazejewska, Ewa

 
9783838113876: Stem cell-based therapy of ocular surface diseases: Novel approaches to expansion of adult epithelial stem cells in culture
Couverture souple
ISBN 10 : 383811387X ISBN 13 : 9783838113876
Editeur : Suedwestdeutscher Verlag fuer Hochschulschriften, 2010

Synopsis

One emerging surgical strategy for restoring a normal corneal epithelial surface in patients with limbal stem cell (SC) deficiency is transplantation of ex vivo expanded limbal epithelial SC which represents one of the few adult human SC therapies being currently employed. In unilaterally affected patients, this therapeutic approach commonly involves the harvest of a small limbal biopsy from the patient´s contralateral healthy eye followed by cell expansion ex vivo and generation of an epithelial sheet on a transplantable carrier. Although successful restoration of the ocular surface occurs in the early postoperative period, the long-term outcome is less satisfactory, most probably due to depletion of limbal SC in culture. In this work an improved culture protocol was established for isolation, enrichment and preservation of limbal epithelial stem and progenitor cells within transplantable cultivated epithelial cell sheets. Furthermore, the work focused on the examination of hair follicles as an alternative autologous SC source and developement of a new more promising therapy for patients with bilateral limbal SC deficiency.

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Présentation de l'éditeur

One emerging surgical strategy for restoring a normal corneal epithelial surface in patients with limbal stem cell (SC) deficiency is transplantation of ex vivo expanded limbal epithelial SC which represents one of the few adult human SC therapies being currently employed. In unilaterally affected patients, this therapeutic approach commonly involves the harvest of a small limbal biopsy from the patient´s contralateral healthy eye followed by cell expansion ex vivo and generation of an epithelial sheet on a transplantable carrier. Although successful restoration of the ocular surface occurs in the early postoperative period, the long-term outcome is less satisfactory, most probably due to depletion of limbal SC in culture. In this work an improved culture protocol was established for isolation, enrichment and preservation of limbal epithelial stem and progenitor cells within transplantable cultivated epithelial cell sheets. Furthermore, the work focused on the examination of hair follicles as an alternative autologous SC source and developement of a new more promising therapy for patients with bilateral limbal SC deficiency.

Biographie de l'auteur

Geboren am 31.01.1979 in Tarnów (Polen) 2000-2004 Studium der Biologie an der Friedrich-Alexander-Universität Erlangen-Nürnberg 2005 Diplomarbeit am Institut für Klinische und Molekulare Virologie der Friedrich-Alexander-Universität Erlangen-Nürnberg 2006-2009 Promotion in der Augenklinik der Friedrich-Alexander-Universität Erlangen Nürnberg

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Suedwestdeutscher Verlag fuer Hochschulschriften
Date d'édition
2010
Langue
anglais
ISBN 10 
383811387X
ISBN 13 
9783838113876
Reliure
Broché
Nombre de pages
84
Coordonnées du fabricant
non disponible
Personne responsable
info@bod.de
info@bod.de

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