Microparticle Formation by Spray-Freeze-Drying: The Influence of Atomization Conditions on Protein Secondary and Tertiary Structure - Couverture souple

Vonhoff, Sebastian

 
9783838117713: Microparticle Formation by Spray-Freeze-Drying: The Influence of Atomization Conditions on Protein Secondary and Tertiary Structure
Couverture souple
ISBN 10 : 3838117719 ISBN 13 : 9783838117713
Editeur : Suedwestdeutscher Verlag fuer Hochschulschriften, 2010

Synopsis

This thesis deals with the preparation microparticles by spray-freeze-drying (SFD). As the process consists of various unit operations, the stability of labile APIs, such as proteins, can be serverly impaired. The secondary structure of a protein is known to be directly linked to its overall stability. However, quantification of protein secondary structure by FTIR spectroscopy can show poor reproducibility. Therefore, the first part of this thesis covers the development of a new FTIR method for objective and fast determination of protein secondary structure. The second part investigates the influence of atomization conditions on protein secondary and tertiary structure as well as residual enzyme activity during SFD. The process is analyzed after a) atomization b) atomization, freezing and thawing and c) the complete SFD process. A thorough comparison of the utilized ultrasound nozzles (including particle size distribution, temperatures and cavitation effects) is performed in the third part of this thesis. In the fourth and last part, different formulations containing a mixture of alpha-chymotrypsin and various excipients are evaluated for their stability.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

This thesis deals with the preparation microparticles by spray-freeze-drying (SFD). As the process consists of various unit operations, the stability of labile APIs, such as proteins, can be serverly impaired. The secondary structure of a protein is known to be directly linked to its overall stability. However, quantification of protein secondary structure by FTIR spectroscopy can show poor reproducibility. Therefore, the first part of this thesis covers the development of a new FTIR method for objective and fast determination of protein secondary structure. The second part investigates the influence of atomization conditions on protein secondary and tertiary structure as well as residual enzyme activity during SFD. The process is analyzed after a) atomization b) atomization, freezing and thawing and c) the complete SFD process. A thorough comparison of the utilized ultrasound nozzles (including particle size distribution, temperatures and cavitation effects) is performed in the third part of this thesis. In the fourth and last part, different formulations containing a mixture of alpha-chymotrypsin and various excipients are evaluated for their stability.

Biographie de l'auteur

Sebastian Vonhoff passed his licensing exam as German pharmacist in 2006. From 2006 - 2009 he did his PhD thesis under the supervision of Prof. Dr. Geoffrey Lee at the Division of Pharmaceutics (University Erlangen-Nuremberg). Focus laid on analysis of protein secondary structure by FTIR spectroscopy and particle formation by spray-freeze-drying.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Suedwestdeutscher Verlag fuer Hochschulschriften
Date d'édition
2010
Langue
anglais
ISBN 10 
3838117719
ISBN 13 
9783838117713
Reliure
Broché
Nombre de pages
188
Coordonnées du fabricant
non disponible
Personne responsable
non disponible