Quantifying cell-ECM adhesion by single cell force spectroscopy - Couverture souple

Taubenberger, Anna

 
9783838124278: Quantifying cell-ECM adhesion by single cell force spectroscopy
Couverture souple
ISBN 10 : 3838124278 ISBN 13 : 9783838124278
Editeur : Suedwestdeutscher Verlag fuer Hochschulschriften, 2011

Synopsis

Integrin-mediated adhesive interactions of cells with the extracellular matrix (ECM) regulate important cellular functions and play a critical role in disease. Therefore, studying the mechanisms underlying integrin-binding and regulation is of great interest. In the presented work three respective biological questions have been addressed using Atomic Force Microscopy based Single Cell Force Spectroscopy (AFM-SCFS). The work is split into six chapters. The first chapter provides an introduction into cell-ECM interactions. Thereafter different adhesion assays are compared and contrasted, and the theoretical Bell-Evans model is discussed. In chapter four, alpha2beta1-integrin mediated cell adhesion to collagen I is quantified. Thereafter the effects of the exposure of cryptic integrin binding sites within collagen I on pre-osteoblasts are analysed. At last, the role of BCR/ABL in modulating myeloid progenitor cell adhesion to stromal cells and ECM-coated substrates is studied. Taken together, the work demonstrates that AFM-SCFS is a versatile tool that permits monitoring of cell adhesion from single-molecule recognition events to the formation of more complex adhesion sites.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Integrin-mediated adhesive interactions of cells with the extracellular matrix (ECM) regulate important cellular functions and play a critical role in disease. Therefore, studying the mechanisms underlying integrin-binding and regulation is of great interest. In the presented work three respective biological questions have been addressed using Atomic Force Microscopy based Single Cell Force Spectroscopy (AFM-SCFS). The work is split into six chapters. The first chapter provides an introduction into cell-ECM interactions. Thereafter different adhesion assays are compared and contrasted, and the theoretical Bell-Evans model is discussed. In chapter four, alpha2beta1-integrin mediated cell adhesion to collagen I is quantified. Thereafter the effects of the exposure of cryptic integrin binding sites within collagen I on pre-osteoblasts are analysed. At last, the role of BCR/ABL in modulating myeloid progenitor cell adhesion to stromal cells and ECM-coated substrates is studied. Taken together, the work demonstrates that AFM-SCFS is a versatile tool that permits monitoring of cell adhesion from single-molecule recognition events to the formation of more complex adhesion sites.

Biographie de l'auteur

Anna Taubenberger, born 1980 in Kulmbach, Germany, graduated 2004 from the TU Dresden with a degree in Engineering. Between 2005 and 2009 she did her PhD at the Biotechnological Centre Dresden on the presented work. Since 2009 she is working as a DFG postdoctoral research fellow at Queensland University of Technology in Brisbane, Australia.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Suedwestdeutscher Verlag fuer Hochschulschriften
Date d'édition
2011
Langue
anglais
ISBN 10 
3838124278
ISBN 13 
9783838124278
Reliure
Broché
Nombre de pages
184
Coordonnées du fabricant
non disponible
Personne responsable
non disponible