Expanded Bed Adsorption Chromatography: For Purification of Recombinant GST Protein - Couverture souple

Abdullah, Norhafizah

 
9783838310299: Expanded Bed Adsorption Chromatography: For Purification of Recombinant GST Protein
Couverture souple
ISBN 10 : 3838310292 ISBN 13 : 9783838310299
Editeur : LAP Lambert Academic Publishing, 2009

Synopsis

Purifying protein is often a complicated task. This is due to protein?s heterogeneity, complexity and instability when not in their native environment. It is the goal of this book to assemble the most generic approach for protein purification. The use of an expanded bed adsorption (EBA) chromatography as an integrative product capture and concentration technique allows single step purification of biomolecules from complex feedstock a possible alternative. There are a number of different approaches that can be taken in the choice and design of an appropriate adsorbent for use in EBA procedure. In this book, experimental investigation was focused on the choice of the ligand to be used on the solid phase adsorbent to ensure selective isolation of the target protein from a complex feedstock. True biospecific affinity, immobilized metal affinity chelating and ion exchange strategies were investigated for EBA purification. Recombinant glutathione-S-transferase (GST) expressed intracellularly in E.coli was chosen as a model protein. The book is aimed at researchers working in the area of protein purification.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Purifying protein is often a complicated task. This is due to protein?s heterogeneity, complexity and instability when not in their native environment. It is the goal of this book to assemble the most generic approach for protein purification. The use of an expanded bed adsorption (EBA) chromatography as an integrative product capture and concentration technique allows single step purification of biomolecules from complex feedstock a possible alternative. There are a number of different approaches that can be taken in the choice and design of an appropriate adsorbent for use in EBA procedure. In this book, experimental investigation was focused on the choice of the ligand to be used on the solid phase adsorbent to ensure selective isolation of the target protein from a complex feedstock. True biospecific affinity, immobilized metal affinity chelating and ion exchange strategies were investigated for EBA purification. Recombinant glutathione-S-transferase (GST) expressed intracellularly in E.coli was chosen as a model protein. The book is aimed at researchers working in the area of protein purification.

Biographie de l'auteur

Dr. Norhafizah Abdullah gained her PhD in Chemical Engineering (Kings College, Cambridge, UK) and MSc in Bioreactor Systems (UMIST,UK) before entering her current profession as an Associate Professor at Universiti Putra Malaysia, Malaysia. Her current research topics include protein purification, drug delivery and bioremediation.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP Lambert Academic Publishing
Date d'édition
2009
Langue
anglais
ISBN 10 
3838310292
ISBN 13 
9783838310299
Reliure
Broché
Nombre de pages
272
Coordonnées du fabricant
non disponible
Personne responsable
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