Articles liés à Development of a Collagen Gel Sandwich Hepatocyte Bioreactor...
Development of a Collagen Gel Sandwich Hepatocyte Bioreactor: In Vitro Detection of Hepatotoxicity Caused by Drugs and Chemicals - Couverture souple
Synopsis
Understanding the hepatotoxicity of drugs is essential for progress in academic and industrial research. Studying hepatotoxicity in vitro is challenging because hepatocytes, the metabolically active cells of the liver, are very difficult to maintain in culture. It has been observed that culturing hepatocytes between two layers of collagen maintains in vivo-like morphology and also many drug metabolizing enzymes for weeks. In spite of the research examining drug metabolism in collagen sandwiches, there is limited data regarding the applicability of this system to hepatotoxicity studies. We cultured primary rat hepatocytes in the collagen sandwich configuration and optimized this system for long-term hepatotoxicity studies. In addition, we also examined the conditioned medium from the hepatocytes to investigate the secreted protein profile, which could potentially be used to find toxicity biomarkers. Our proteomics analysis combined gel electrophoresis with Qstar LC-MS/MS, and we were able to identify 493 proteins. In summary, we have shown that the collagen sandwich is a suitable system for hepatotoxicity studies as well as proteomics analysis.
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
Présentation de l'éditeur
Understanding the hepatotoxicity of drugs is essential for progress in academic and industrial research. Studying hepatotoxicity in vitro is challenging because hepatocytes, the metabolically active cells of the liver, are very difficult to maintain in culture. It has been observed that culturing hepatocytes between two layers of collagen maintains in vivo-like morphology and also many drug metabolizing enzymes for weeks. In spite of the research examining drug metabolism in collagen sandwiches, there is limited data regarding the applicability of this system to hepatotoxicity studies. We cultured primary rat hepatocytes in the collagen sandwich configuration and optimized this system for long-term hepatotoxicity studies. In addition, we also examined the conditioned medium from the hepatocytes to investigate the secreted protein profile, which could potentially be used to find toxicity biomarkers. Our proteomics analysis combined gel electrophoresis with Qstar LC-MS/MS, and we were able to identify 493 proteins. In summary, we have shown that the collagen sandwich is a suitable system for hepatotoxicity studies as well as proteomics analysis.
Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.
Résultats de recherche pour Development of a Collagen Gel Sandwich Hepatocyte Bioreactor...
Image fournie par le vendeur
Development of a Collagen Gel Sandwich Hepatocyte Bioreactor
Edité par
LAP LAMBERT Academic Publishing Mrz 2010, 2010
ISBN 10 : 3838350162
ISBN 13 : 9783838350165
Neuf
Taschenbuch
Vendeur : BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Allemagne
Évaluation du vendeur 5 sur 5 étoiles
Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Understanding the hepatotoxicity of drugs is essential for progress in academic and industrial research. Studying hepatotoxicity in vitro is challenging because hepatocytes, the metabolically active cells of the liver, are very difficult to maintain in culture. It has been observed that culturing hepatocytes between two layers of collagen maintains in vivo-like morphology and also many drug metabolizing enzymes for weeks. In spite of the research examining drug metabolism in collagen sandwiches, there is limited data regarding the applicability of this system to hepatotoxicity studies. We cultured primary rat hepatocytes in the collagen sandwich configuration and optimized this system for long-term hepatotoxicity studies. In addition, we also examined the conditioned medium from the hepatocytes to investigate the secreted protein profile, which could potentially be used to find toxicity biomarkers. Our proteomics analysis combined gel electrophoresis with Qstar LC-MS/MS, and we were able to identify 493 proteins. In summary, we have shown that the collagen sandwich is a suitable system for hepatotoxicity studies as well as proteomics analysis. 156 pp. Englisch. N° de réf. du vendeur 9783838350165
Acheter neuf
EUR 59
Expédition à EUR 23
Expédition depuis Allemagne vers Etats-Unis
Expédition depuis Allemagne vers Etats-Unis
Quantité disponible : 2 disponible(s)
Image fournie par le vendeur
Development of a Collagen Gel Sandwich Hepatocyte Bioreactor
Edité par
LAP LAMBERT Academic Publishing, 2010
ISBN 10 : 3838350162
ISBN 13 : 9783838350165
Neuf
Couverture souple
Vendeur : moluna, Greven, Allemagne
Évaluation du vendeur 5 sur 5 étoiles
Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Farkas DoraDora Farkas, Ph.D., completed both her Bachelor s degree inChemical Engineering and her Ph.D. in Toxicology at theMassachusetts Institute of Technology. After she earned herPh.D. she worked as a postdoctoral fellow in the . N° de réf. du vendeur 5415438
Acheter neuf
EUR 48,50
Expédition à EUR 48,99
Expédition depuis Allemagne vers Etats-Unis
Expédition depuis Allemagne vers Etats-Unis
Quantité disponible : Plus de 20 disponibles
Image fournie par le vendeur
Development of a Collagen Gel Sandwich Hepatocyte Bioreactor : In Vitro Detection of Hepatotoxicity Caused by Drugs and Chemicals
Edité par
LAP LAMBERT Academic Publishing, 2010
ISBN 10 : 3838350162
ISBN 13 : 9783838350165
Neuf
Taschenbuch
Vendeur : AHA-BUCH GmbH, Einbeck, Allemagne
Évaluation du vendeur 5 sur 5 étoiles
Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Understanding the hepatotoxicity of drugs is essential for progress in academic and industrial research. Studying hepatotoxicity in vitro is challenging because hepatocytes, the metabolically active cells of the liver, are very difficult to maintain in culture. It has been observed that culturing hepatocytes between two layers of collagen maintains in vivo-like morphology and also many drug metabolizing enzymes for weeks. In spite of the research examining drug metabolism in collagen sandwiches, there is limited data regarding the applicability of this system to hepatotoxicity studies. We cultured primary rat hepatocytes in the collagen sandwich configuration and optimized this system for long-term hepatotoxicity studies. In addition, we also examined the conditioned medium from the hepatocytes to investigate the secreted protein profile, which could potentially be used to find toxicity biomarkers. Our proteomics analysis combined gel electrophoresis with Qstar LC-MS/MS, and we were able to identify 493 proteins. In summary, we have shown that the collagen sandwich is a suitable system for hepatotoxicity studies as well as proteomics analysis. N° de réf. du vendeur 9783838350165
Acheter neuf
EUR 66,55
Expédition à EUR 35
Expédition depuis Allemagne vers Etats-Unis
Expédition depuis Allemagne vers Etats-Unis
Quantité disponible : 1 disponible(s)
Image fournie par le vendeur
Development of a Collagen Gel Sandwich Hepatocyte Bioreactor
Edité par
LAP LAMBERT Academic Publishing Mär 2010, 2010
ISBN 10 : 3838350162
ISBN 13 : 9783838350165
Neuf
Taschenbuch
Vendeur : buchversandmimpf2000, Emtmannsberg, BAYE, Allemagne
Évaluation du vendeur 5 sur 5 étoiles
Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -Understanding the hepatotoxicity of drugs is essential for progress in academic and industrial research. Studying hepatotoxicity in vitro is challenging because hepatocytes, the metabolically active cells of the liver, are very difficult to maintain in culture. It has been observed that culturing hepatocytes between two layers of collagen maintains in vivo-like morphology and also many drug metabolizing enzymes for weeks. In spite of the research examining drug metabolism in collagen sandwiches, there is limited data regarding the applicability of this system to hepatotoxicity studies. We cultured primary rat hepatocytes in the collagen sandwich configuration and optimized this system for long-term hepatotoxicity studies. In addition, we also examined the conditioned medium from the hepatocytes to investigate the secreted protein profile, which could potentially be used to find toxicity biomarkers. Our proteomics analysis combined gel electrophoresis with Qstar LC-MS/MS, and we were able to identify 493 proteins. In summary, we have shown that the collagen sandwich is a suitable system for hepatotoxicity studies as well as proteomics analysis.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 156 pp. Englisch. N° de réf. du vendeur 9783838350165
Acheter neuf
EUR 59
Expédition à EUR 60
Expédition depuis Allemagne vers Etats-Unis
Expédition depuis Allemagne vers Etats-Unis
Quantité disponible : 1 disponible(s)
Image fournie par le vendeur
Development of a Collagen Gel Sandwich Hepatocyte Bioreactor | In Vitro Detection of Hepatotoxicity Caused by Drugs and Chemicals
Edité par
LAP LAMBERT Academic Publishing, 2010
ISBN 10 : 3838350162
ISBN 13 : 9783838350165
Neuf
Taschenbuch
Vendeur : preigu, Osnabrück, Allemagne
Évaluation du vendeur 5 sur 5 étoiles
Taschenbuch. Etat : Neu. Development of a Collagen Gel Sandwich Hepatocyte Bioreactor | In Vitro Detection of Hepatotoxicity Caused by Drugs and Chemicals | Dora Farkas | Taschenbuch | 156 S. | Englisch | 2010 | LAP LAMBERT Academic Publishing | EAN 9783838350165 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. N° de réf. du vendeur 101216732
Acheter neuf
EUR 51,10
Expédition à EUR 70
Expédition depuis Allemagne vers Etats-Unis
Expédition depuis Allemagne vers Etats-Unis
Quantité disponible : 5 disponible(s)