Fourier Transform Infrared (FT-IR) Spectroscopy: Fourier Transform Infrared (FT-IR) Spectroscopy, A Novel Technique to Study the Biochemical structure of Bacterial Cells - Couverture souple

Al-Qadiri, Hamzah

 
9783838360607: Fourier Transform Infrared (FT-IR) Spectroscopy: Fourier Transform Infrared (FT-IR) Spectroscopy, A Novel Technique to Study the Biochemical structure of Bacterial Cells

Synopsis

This research work was performed to examine the feasibility of using Fourier transform infrared (FT-IR) spectroscopy (in the mid-IR range, 4000-400 cm-1) to i) detect and identify bacterial cells. The greatest spectral variation was observed between 1800 cm-1 and 900 cm-1: for amide I band at ~1650 cm-1, amide II band at ~1540 cm-1, CH3 and CH2 of protein components around 1455 cm-1 and 1398 cm-1, P=O of the phosphodiester backbone of nucleic acids around 1242 cm-1 and 1080 cm-1, and polysaccharide compounds around 1050 - 950 cm-1. From the second derivative transformation and loadings plot, FT-IR showed the potential to detect sublethally injured and dead cells and discriminate between different types and degrees of cell injury.

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Présentation de l'éditeur

This research work was performed to examine the feasibility of using Fourier transform infrared (FT-IR) spectroscopy (in the mid-IR range, 4000-400 cm-1) to i) detect and identify bacterial cells. The greatest spectral variation was observed between 1800 cm-1 and 900 cm-1: for amide I band at ~1650 cm-1, amide II band at ~1540 cm-1, CH3 and CH2 of protein components around 1455 cm-1 and 1398 cm-1, P=O of the phosphodiester backbone of nucleic acids around 1242 cm-1 and 1080 cm-1, and polysaccharide compounds around 1050 - 950 cm-1. From the second derivative transformation and loadings plot, FT-IR showed the potential to detect sublethally injured and dead cells and discriminate between different types and degrees of cell injury.

Biographie de l'auteur

My name is Hamzah Al-Qadiri and I am from Amman-Jordan. I am currently working as assistant professor at the University of Jordan. I obtained my Ph.D. degree from Washington State University. My research work emphasizing on studying novel technique for detection and identification of food and water pathogens using infrared spectroscopy.

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