Application of Microarray technology: A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation. - Couverture souple

Ray, Anamika

 
9783838364063: Application of Microarray technology: A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation.
Couverture souple
ISBN 10 : 3838364066 ISBN 13 : 9783838364063
Editeur : LAP LAMBERT Academic Publishing, 2010

Synopsis

The construction of cDNA libraries is useful in understanding gene expression in organisms under different conditions, but random sequencing of unbiased cDNAs is laborious and gives rise to redundancy. We aimed to isolate cDNAs induced by switching Aspergillus nidulans from glucose to selected polysaccharides. We devised a Negative Subtraction Hybridization method which involved screening a library made from cDNAs from cells grown under a selected physiological condition with labeled cDNA probes from another physiological condition. In a screen for transcripts induced by switching the carbon source, 3,532 negatives were isolated. Single-channel microarray experiments were done to confirm that the majority of the negatives were indeed induced differentially. To gain insight into the regulation, we used microarrays to identify gene expression changes following a shift from glucose to pectin or glucose starvation. The findings from this study portray the useful utilization of the powerful microarray technology to study gene expression profiling under various physiological conditions.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

The construction of cDNA libraries is useful in understanding gene expression in organisms under different conditions, but random sequencing of unbiased cDNAs is laborious and gives rise to redundancy. We aimed to isolate cDNAs induced by switching Aspergillus nidulans from glucose to selected polysaccharides. We devised a Negative Subtraction Hybridization method which involved screening a library made from cDNAs from cells grown under a selected physiological condition with labeled cDNA probes from another physiological condition. In a screen for transcripts induced by switching the carbon source, 3,532 negatives were isolated. Single-channel microarray experiments were done to confirm that the majority of the negatives were indeed induced differentially. To gain insight into the regulation, we used microarrays to identify gene expression changes following a shift from glucose to pectin or glucose starvation. The findings from this study portray the useful utilization of the powerful microarray technology to study gene expression profiling under various physiological conditions.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2010
Langue
anglais
ISBN 10 
3838364066
ISBN 13 
9783838364063
Reliure
Broché
Nombre de pages
68
Coordonnées du fabricant
non disponible
Personne responsable
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