Opto-biochips for Microcytometry: Author's summarizes his research work in the areas of Microfluidics and Photonics - Couverture souple

Goel, Sanket

Couverture souple

ISBN 10 : 383838959X ISBN 13 : 9783838389592

Editeur : LAP LAMBERT Academic Publishing, 2010

Synopsis

Microscale fluorescence activated cell-sorting (micro-FACS) is a technology to identify and separate cells. Different elements of the micro-FACS, fabrication of opto-biochips, particle detection and velocity measurement, and flow switching, are studied in the present work. Biochips were fabricated by casting polydimethylsiloxane (PDMS) on the masters fabricated by direct laser-writing, and standard lithography and dry etching. In a different approach, biochips with integrated waveguides and microchannels were fabricated photolithographically on glass substrates using a negative photoresist, SU-8. In these opto-biochips, two excitation waveguides carrying two different wavelengths intersect the microchannel at two points separated and one large output waveguide carry fluorescence signals to a single filtered detector. The velocities of the microparticles were determined by the captured fluorescence from the double peak. Simulations based on beam propagation method were used to predict the performance of the SU-8 waveguides and the intensity at the two intersections has been compared with the experimental results.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2010
• Langue: anglais
• ISBN 10: 383838959X
• ISBN 13: 9783838389592
• Reliure: Broché
• Nombre de pages: 156
• Coordonnées du fabricant: non disponible
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