RNA Interference in insect cells infected with baculovirus: A universal transgene silencing approach - Couverture souple

Salem, Tamer

 
9783843351355: RNA Interference in insect cells infected with baculovirus: A universal transgene silencing approach
Couverture souple
ISBN 10 : 384335135X ISBN 13 : 9783843351355
Editeur : LAP LAMBERT Academic Publishing, 2010

Synopsis

Baculovirus?insect cell system (BICS) is considered one of the most efficient eukaryotic gene expression systems. Establishing a universal gene silencing (UGS) system is very important due to the increasing number of studies using RNA interference (RNAi) with BICS. In this work, the enhanced green fluorescent protein (EGFP) was used as the RNAi consistent target sequence located downstream of a depressant insect-neurotoxin gene, LqqIT2 used as a model of the gene of interest. Small interfering RNA (siRNA) and inverted repeats of EGFP gene (IR-EG) were examined in targeting the EGFP-LqqIT2 (EL)-fusion mRNA or LqqIT2-EGFP (LE)-bicistronic mRNA for degradation. Suppression efficiencies using these inducers were examined transiently and stably in uninfected and infected insect Sf9 cells.

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Présentation de l'éditeur

Baculovirus?insect cell system (BICS) is considered one of the most efficient eukaryotic gene expression systems. Establishing a universal gene silencing (UGS) system is very important due to the increasing number of studies using RNA interference (RNAi) with BICS. In this work, the enhanced green fluorescent protein (EGFP) was used as the RNAi consistent target sequence located downstream of a depressant insect-neurotoxin gene, LqqIT2 used as a model of the gene of interest. Small interfering RNA (siRNA) and inverted repeats of EGFP gene (IR-EG) were examined in targeting the EGFP-LqqIT2 (EL)-fusion mRNA or LqqIT2-EGFP (LE)-bicistronic mRNA for degradation. Suppression efficiencies using these inducers were examined transiently and stably in uninfected and infected insect Sf9 cells.

Biographie de l'auteur

Tamer Salem is a Research Associate Professor in Molecular Virology. He is working in AGERI, Egypt. He obtained his Ph.D. from Microbiology Department in University of Florida, USA. He had varies post- doctoral and research associate positions in Miami University (Ohio), University of Florida (Florida), and Michigan State University (East Lansing).

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2010
Langue
anglais
ISBN 10 
384335135X
ISBN 13 
9783843351355
Reliure
Broché
Nombre de pages
144
Coordonnées du fabricant
non disponible
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