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Isolation and characterization of novel genes from tomato: Molecular Cloning of genes associated with lycopene biosynthesis and Disease resistance in tomato - Couverture souple

Eswaramoorthy, Hemaprabha

 
9783843354301: Isolation and characterization of novel genes from tomato: Molecular Cloning of genes associated with lycopene biosynthesis and Disease resistance in tomato
Couverture souple
ISBN 10 : 3843354308 ISBN 13 : 9783843354301
Editeur : LAP LAMBERT Academic Publishing, 2010

Synopsis

In the present study, useful genes were isolated and characterized from the wild species of tomato. Biochemical analysis of different wild species, varieties and hybrids of tomato inferred that the wild species, L. pimpinellifolium is to be a potential source for lycopene. Hence partial cDNA coding for a key enzyme in lycopene biosynthesis, phytoene desaturase (PDS) was isolated by RT-PCR from L. pimpinellifolium. Artificial screening of tomato wild species and cultivars for resistance to Tospovirus, Fusarium and Root knot nematode revealed that L. peruvianum was resistant to all. cDNAs of three R genes coding for Tospovirus, Fusarium and Root knot nematode resistance were isolated from the wild species, L. peruvianum. A cDNA of R gene involved in Fusarium resistance was isolated from L. pimpinellifolium. Sequence analysis using bioinformatics tools viz, LALIGN, BALST, BLASTp, ClustalW, Treetop, ScanProsite of all the four cDNAs isolated corresponding to the different R genes showed considerable homology with already available disease / nematode resistance genes.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

In the present study, useful genes were isolated and characterized from the wild species of tomato. Biochemical analysis of different wild species, varieties and hybrids of tomato inferred that the wild species, L. pimpinellifolium is to be a potential source for lycopene. Hence partial cDNA coding for a key enzyme in lycopene biosynthesis, phytoene desaturase (PDS) was isolated by RT-PCR from L. pimpinellifolium. Artificial screening of tomato wild species and cultivars for resistance to Tospovirus, Fusarium and Root knot nematode revealed that L. peruvianum was resistant to all. cDNAs of three R genes coding for Tospovirus, Fusarium and Root knot nematode resistance were isolated from the wild species, L. peruvianum. A cDNA of R gene involved in Fusarium resistance was isolated from L. pimpinellifolium. Sequence analysis using bioinformatics tools viz, LALIGN, BALST, BLASTp, ClustalW, Treetop, ScanProsite of all the four cDNAs isolated corresponding to the different R genes showed considerable homology with already available disease / nematode resistance genes.

Biographie de l'auteur

Dr. Hemaprabha Eswaramoorthy is a Research Associate in Wayne State University, Detroit, Michigan. She obtained her Msc and Ph.D in Biotechnology from Tamilnadu Agricultural University.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2010
Langue
anglais
ISBN 10 
3843354308
ISBN 13 
9783843354301
Reliure
Broché
Nombre de pages
176
Coordonnées du fabricant
non disponible
Personne responsable
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