Electrochemical synchronization of cell spreading on solid surfaces: Dictyostelium discoideum: synchronization of spreading - Couverture souple

Socol, Marius

 
9783843360630: Electrochemical synchronization of cell spreading on solid surfaces: Dictyostelium discoideum: synchronization of spreading
Couverture souple
ISBN 10 : 3843360634 ISBN 13 : 9783843360630
Editeur : LAP LAMBERT Academic Publishing, 2010

Synopsis

Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion. LimE- GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method.

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Présentation de l'éditeur

Cell synchronization is important for the analysis of molecular events involved in cell spreading and motility. Electrostatic interactions between cells and surfaces were investigated in order to synchronize the first step in cell adhesion. LimE- GFP marked Dictyostelium discoideum cells were used for fluorescent tracking of actin polymerization events. Oscillating LimE fluorescent peaks were observed for individual cells in standard phosphate buffer during spreading. At low ionic concentration (phosphate sucrose buffer 0.17 mM), cells levitate over the conductive surfaces (Indium Tin Oxide, ITO) due to electrostatic repulsion. An electrochemical device was designed in order to apply an overpotential pulse (+2.5 V/Ag,AgCl) during 0.1 s to the ITO surface. In these conditions, protons are produced by water oxidation, which reduce the ITO negative surface charge and thus, attracting the levitating cells simultaneously. Consequently, these irreversible contacts with the surface triggered the onset of cell spreading. Therefore, we obtained synchronization of the spreading of a cell population for the first time thanks to an electrochemical method.

Biographie de l'auteur

Marius Socol, PhD in Biophysics (2010) Grenoble Joseph Fourier University and PhD in Chemistry (2008) Cluj-Napoca Babe?-Bolyai University: studied Physics and Chemistry at Babe? Bolyai University of Cluj-Napoca. PostDoc research scientist at INSERM, U600, Luminy, Marseille.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2010
Langue
anglais
ISBN 10 
3843360634
ISBN 13 
9783843360630
Reliure
Broché
Nombre de pages
168
Coordonnées du fabricant
non disponible
Personne responsable
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