Functional Genomic Analysis of Pseudorabies Virus: Functional Analysis of Pseudorabies Virus Genes by Real-Time RT PCR and Knock-Out Technique - Couverture souple

Tombacz, Dora; Boldogkoi, Zsolt

 
9783843389488: Functional Genomic Analysis of Pseudorabies Virus: Functional Analysis of Pseudorabies Virus Genes by Real-Time RT PCR and Knock-Out Technique
Couverture souple
ISBN 10 : 3843389489 ISBN 13 : 9783843389488
Editeur : LAP LAMBERT Academic Publishing, 2011

Synopsis

The pseudorabies virus (PRV) is a neurotropic herpesvirus, it is a pathogen of swine, causing Aujeszky's disease. PRV is a widely used model organism for studies in pathogenesis and molecular biology. This virus is also utilized as a neural circuit tracer. We have developed (1) a real-time RT-PCR method for the global analysis of PRV gene expression; (2) a novel calculation method to examine the relative expression ratios, and which is applicable for the analysis of gene expression in any genetic system that progressively changes in time; (3) PRV-based troponeon expressing transsynaptic tracers which label synaptically connected nerve cells; (4) transgenic viruses encoding differentially colored fluorescent proteins (FPs) and multiple colored FPs to differentiate the brain regions; (5) a method for a short-term culture of isolated cardiac muscle cells that retain their morphological and physiological integrity; (6) a PRV-based system for delivery of foreign genes to cardiomyocytes.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

The pseudorabies virus (PRV) is a neurotropic herpesvirus, it is a pathogen of swine, causing Aujeszky's disease. PRV is a widely used model organism for studies in pathogenesis and molecular biology. This virus is also utilized as a neural circuit tracer. We have developed (1) a real-time RT-PCR method for the global analysis of PRV gene expression; (2) a novel calculation method to examine the relative expression ratios, and which is applicable for the analysis of gene expression in any genetic system that progressively changes in time; (3) PRV-based troponeon expressing transsynaptic tracers which label synaptically connected nerve cells; (4) transgenic viruses encoding differentially colored fluorescent proteins (FPs) and multiple colored FPs to differentiate the brain regions; (5) a method for a short-term culture of isolated cardiac muscle cells that retain their morphological and physiological integrity; (6) a PRV-based system for delivery of foreign genes to cardiomyocytes.

Biographie de l'auteur

Dora Tombacz & Zsolt Boldogkoi work in the Department of Medical Biology, University of Szeged (HUN). Prof. Zsolt Boldogkoi (med.habil) is the Head of the Department of Medical Biology. He is MSc in Biology and Economics, PhD and DSc in Molecular Biology. Dora is MSc in Biology and PhD in Medical Sciences. She is a member of Boldogkoi group.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2011
Langue
anglais
ISBN 10 
3843389489
ISBN 13 
9783843389488
Reliure
Broché
Nombre de pages
76
Coordonnées du fabricant
non disponible
Personne responsable
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