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CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY: FROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY - Couverture souple

Wu, Jiaqian

 
9783844320602: CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY: FROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY
Couverture souple
ISBN 10 : 3844320601 ISBN 13 : 9783844320602
Editeur : LAP LAMBERT Academic Publishing, 2011

Synopsis

In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully identified and experimentally verified novel mammalian genes such as full-length rat homologs of human disease genes. Furthermore, I studied transcriptome complexity including SNPs and alternative splicing in leukemia samples using quantitative PCR, and we developed a trace analysis technology to identify multiple alternative spliced transcripts in one single sequencing reaction. My results suggested that alternatively spliced transcripts show cancer type specific up- or down- regulation, and the ratio of alternative spliced isoforms may underlie the cancer mechanism.

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Présentation de l'éditeur

In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully identified and experimentally verified novel mammalian genes such as full-length rat homologs of human disease genes. Furthermore, I studied transcriptome complexity including SNPs and alternative splicing in leukemia samples using quantitative PCR, and we developed a trace analysis technology to identify multiple alternative spliced transcripts in one single sequencing reaction. My results suggested that alternatively spliced transcripts show cancer type specific up- or down- regulation, and the ratio of alternative spliced isoforms may underlie the cancer mechanism.

Biographie de l'auteur

Jiaqian Wu, Ph.D. authored many peer-reviewed publications in the field of Genetics, Genomics and Stem Cell research. She won multiple awards and has one patent. Jiaqian has given talks at international conferences and research institutes. Jiaqian is currently an assistant professor at the University of Texas Medical School at Houston.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2011
Langue
anglais
ISBN 10 
3844320601
ISBN 13 
9783844320602
Reliure
Broché
Nombre de pages
204
Coordonnées du fabricant
non disponible
Personne responsable
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