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Gut microflora and diet impacts on human colonic adenocarcinoma cells: Molecular studies on the influence of gut microflora and short-chain fatty acids on apoptosis in human colon cancer cells - Couverture souple

Altonsy, Mohammed O

 
9783845403465: Gut microflora and diet impacts on human colonic adenocarcinoma cells: Molecular studies on the influence of gut microflora and short-chain fatty acids on apoptosis in human colon cancer cells
Couverture souple
ISBN 10 : 3845403462 ISBN 13 : 9783845403465
Editeur : LAP LAMBERT Academic Publishing, 2020

Synopsis

The induction of apoptosis in mammalian cells by bacteria is well reported. This process may assist infection by pathogens whereas for non-pathogens apoptosis induction within carcinoma cells protects against colon cancer. Here, apoptosis induction by a major new gut bacterium, Atopobium minutum, was compared with induction by commensal (Escherichia coli K-12 strains), probiotic (Lactobacillus rhamnosus, Bifidobacterium latis) and pathogenic (E. coli: EPEC and VTEC) gut bacteria within the colon cancer cell line, Caco-2. The results show a major apoptotic effect for the pathogens, mild effects for the probiotic strains and A. minutum, but no effect for commensal E. coli. Short chain fatty acids (SCFAs) are the main fermentation products of intestinal saccharolytic bacteria in the colon and are thought to protect against colon cancer by inducing apoptosis in transformed cells. Our results confirmed that SCFAs caused morphological changes in human colonic carcinoma cell line (Caco-2) indicative of apoptosis. Analysis of various molecular markers of apoptosis (Bcl-2, caspases, cytochrome c release and cell membrane FAS levels) strongly indicated that effect.

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Présentation de l'éditeur

The induction of apoptosis in mammalian cells by bacteria is well reported. This process may assist infection by pathogens whereas for non-pathogens apoptosis induction within carcinoma cells protects against colon cancer. Here, apoptosis induction by a major new gut bacterium, Atopobium minutum, was compared with induction by commensal (Escherichia coli K-12 strains), probiotic (Lactobacillus rhamnosus, Bifidobacterium latis) and pathogenic (E. coli: EPEC and VTEC) gut bacteria within the colon cancer cell line, Caco-2. The results show a major apoptotic effect for the pathogens, mild effects for the probiotic strains and A. minutum, but no effect for commensal E. coli. Short chain fatty acids (SCFAs) are the main fermentation products of intestinal saccharolytic bacteria in the colon and are thought to protect against colon cancer by inducing apoptosis in transformed cells. Our results confirmed that SCFAs caused morphological changes in human colonic carcinoma cell line (Caco-2) indicative of apoptosis. Analysis of various molecular markers of apoptosis (Bcl-2, caspases, cytochrome c release and cell membrane FAS levels) strongly indicated that effect.

Biographie de l'auteur

Dr. Mohammed O AltonsyPhD. “Molecular Biology” University of Reading, UK.Lecturer of Molecular Biology, Sohag Faculty of Sciences, Sohag UniversityContact DetailsEmail:moaltonsy@gmail.comTelephone:+2 (0) 93 4602965- internal 2536 or 2156 Fax:+2 (0) 93 601159Building:Sohag University - Faculty of Science, 2nd floor, Room 126- 285224

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Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2020
Langue
anglais
ISBN 10 
3845403462
ISBN 13 
9783845403465
Reliure
Broché
Nombre de pages
248
Coordonnées du fabricant
non disponible
Personne responsable
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