Articles liés à Simultaneous estimation of antidiabetic drugs by HPLC:...

Simultaneous estimation of antidiabetic drugs by HPLC: Determination of Metformin HCl, Pioglitazone HCl, and Glimepiride using Reverse Phase Liquid Chromatography - Couverture souple

Amin, Mr.Maulikkumar; Jain, Mr.Deepak Kumar; Jain, Dr Deepti

 
9783845440446: Simultaneous estimation of antidiabetic drugs by HPLC: Determination of Metformin HCl, Pioglitazone HCl, and Glimepiride using Reverse Phase Liquid Chromatography
Couverture souple
ISBN 10 : 3845440449 ISBN 13 : 9783845440446
Editeur : LAP LAMBERT Academic Publishing, 2011

Synopsis

A simple, precise, rapid, and reproducible reversed-phase high-performance liquid chromatography method is developed for the simultaneous estimation of metformin hydrochloride (MET), pioglitazone hydrochloride (PIO), and glimepiride (GLP) present in multicomponent dosage forms. Chromatography is carried out isocratically at 25 degrees C 0.5 degrees C on an Inertsil-ODS-3 (C-18) Column (250 x 4.60 mm, 5 microm) with a mobile phase composed of methanol-phosphate buffer (pH 4.3) in the ratio of 75:25 v/v at a flow rate of 1 mL/min. Detection is carried out using a UV-PDA detector at 258 nm. Parameters such as linearity, precision, accuracy, recovery, specificity, and ruggedness are studied as reported in the International Conference on Harmonization guidelines. The retention times for MET, PIO, and GLP are 2.66 + 0.5 min, 7.12 + 0.5 min, and 10.17 + 0.5 min, respectively. The linearity range and percentage recoveries for MET, PIO, and GLP are 10-5000, 10-150, and 1-10 microg/mL and 100.4%, 100.06%, and 100.2%, respectively. The correlation coefficients for all components are close to 1.

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Présentation de l'éditeur

A simple, precise, rapid, and reproducible reversed-phase high-performance liquid chromatography method is developed for the simultaneous estimation of metformin hydrochloride (MET), pioglitazone hydrochloride (PIO), and glimepiride (GLP) present in multicomponent dosage forms. Chromatography is carried out isocratically at 25 degrees C 0.5 degrees C on an Inertsil-ODS-3 (C-18) Column (250 x 4.60 mm, 5 microm) with a mobile phase composed of methanol-phosphate buffer (pH 4.3) in the ratio of 75:25 v/v at a flow rate of 1 mL/min. Detection is carried out using a UV-PDA detector at 258 nm. Parameters such as linearity, precision, accuracy, recovery, specificity, and ruggedness are studied as reported in the International Conference on Harmonization guidelines. The retention times for MET, PIO, and GLP are 2.66 + 0.5 min, 7.12 + 0.5 min, and 10.17 + 0.5 min, respectively. The linearity range and percentage recoveries for MET, PIO, and GLP are 10-5000, 10-150, and 1-10 microg/mL and 100.4%, 100.06%, and 100.2%, respectively. The correlation coefficients for all components are close to 1.

Biographie de l'auteur

M.Pharm Assistant Professor Department of Pharmaceutical Chemistry Kalol Institute of Pharmacy, B/h Old Janpath Hotel, Kalol-Mehsana Highway,Kalol-382721Gujarat, India.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2011
Langue
anglais
ISBN 10 
3845440449
ISBN 13 
9783845440446
Reliure
Broché
Nombre de pages
116
Coordonnées du fabricant
non disponible
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