Functional Analysis of Promoter of cus Operon: Activity of Copper-mediated Induction of cus Operon Promoter in E. coli. - Couverture souple

Zahid, Nageena; Shakoori, Abdul Rauf

 
9783846505113: Functional Analysis of Promoter of cus Operon: Activity of Copper-mediated Induction of cus Operon Promoter in E. coli.
Couverture souple
ISBN 10 : 3846505110 ISBN 13 : 9783846505113
Editeur : LAP LAMBERT Academic Publishing, 2011

Synopsis

Copper is an essential trace metal for both prokaryotes and eukaryotes, and is associated with various metal enzymes which play many essential roles in the living system. But excess of the metal is very toxic and even lethal for living organisms. In microorganisms the intracellular level of copper is maintained by many genes working as an operon and induced by the presence of copper in their environment. One such operon is called cus operon regulated by cusRS two component system in Klebsiella pneumoniae. This cusS (sensory region) and cusR (regulatory region) autoregulate the expression of structural as well as regulatory part of the cus operon by binding of CusR protein to the bidirectional promoter part of the operon.The functional analysis showed that the cloned promoter was highly sensitive to copper metal concentrations and follows Michaelis Menton kinetics at very high concentrations. Additionally, cusRS promoter was also scanned for various transcriptional factor binding sites like -10, -35 boxes for binding of RNA polymerase. Similarly, CusR binding region was highlighted after docking of CusR protein on promoter sequence via Hex Dock software.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Copper is an essential trace metal for both prokaryotes and eukaryotes, and is associated with various metal enzymes which play many essential roles in the living system. But excess of the metal is very toxic and even lethal for living organisms. In microorganisms the intracellular level of copper is maintained by many genes working as an operon and induced by the presence of copper in their environment. One such operon is called cus operon regulated by cusRS two component system in Klebsiella pneumoniae. This cusS (sensory region) and cusR (regulatory region) autoregulate the expression of structural as well as regulatory part of the cus operon by binding of CusR protein to the bidirectional promoter part of the operon.The functional analysis showed that the cloned promoter was highly sensitive to copper metal concentrations and follows Michaelis Menton kinetics at very high concentrations. Additionally, cusRS promoter was also scanned for various transcriptional factor binding sites like -10, -35 boxes for binding of RNA polymerase. Similarly, CusR binding region was highlighted after docking of CusR protein on promoter sequence via Hex Dock software.

Biographie de l'auteur

Nageena Zahid,M.Phil. is a Lecturer in Institute of Biotechnology, Bahauddin Zakariya University, Multan. Abdul Rauf Shakoori is a Distinguished National Professor and Director, School of Biological Sciences, University of the Punjab. He is Fellow of Academy of Sciences for Developing World (TWAS) and Fellow of Pakistan Academy of Sciences.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2011
Langue
anglais
ISBN 10 
3846505110
ISBN 13 
9783846505113
Reliure
Broché
Nombre de pages
76
Coordonnées du fabricant
non disponible
Personne responsable
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