Articles liés à Hyper Production of Laccases: by Neurospora Sitophila

Hyper Production of Laccases: by Neurospora Sitophila - Couverture souple

Imran, Muhammad; Hadri, Saqib Hussain; Asad, Muhammad Javaid

 
9783846509265: Hyper Production of Laccases: by Neurospora Sitophila
Couverture souple
ISBN 10 : 3846509264 ISBN 13 : 9783846509265
Editeur : LAP LAMBERT Academic Publishing, 2011

Synopsis

The main objective of this research project was the production of laccase under optimum conditions, its partial purification and the effect of UV mutagenesis in Neurospora sitophila for hyper production of laccase. The temperature and pH of the incubation were 28 oC and 5.5 respectively. Growth medium employed to culture the Neurospora sitophila for the production of laccase was optimized through various experiments. The fermentation conditions like incubation period, moisture level, inoculum size, yeast extract and tween-20 were optimized. It was observed that the addition of yeast extract and tween-20 enhanced laccase production by Neurospora sitophila in corn cob. Neurospora sitophila produced maximum laccase 3.995U/mL/min in the growth medium of 70% moisture level, 5mL of the inoculum, 0.4% yeast extract and 0.2% of tween-20. The laccase produced by Neurosopora sitophila under optimized conditions was partially purified using ammonium sulfate precipitation.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

The main objective of this research project was the production of laccase under optimum conditions, its partial purification and the effect of UV mutagenesis in Neurospora sitophila for hyper production of laccase. The temperature and pH of the incubation were 28 oC and 5.5 respectively. Growth medium employed to culture the Neurospora sitophila for the production of laccase was optimized through various experiments. The fermentation conditions like incubation period, moisture level, inoculum size, yeast extract and tween-20 were optimized. It was observed that the addition of yeast extract and tween-20 enhanced laccase production by Neurospora sitophila in corn cob. Neurospora sitophila produced maximum laccase 3.995U/mL/min in the growth medium of 70% moisture level, 5mL of the inoculum, 0.4% yeast extract and 0.2% of tween-20. The laccase produced by Neurosopora sitophila under optimized conditions was partially purified using ammonium sulfate precipitation.

Biographie de l'auteur

Muhammad Imran has worked as teacher lin Biochemistry and working at the Department of Biochemistry, PMAS Arid Agriculture University Rawalpindi, Pakistan. Saqib Hussain Hadri has a Ph.D degree in Biochemistry from PMAS Arid Agriculture University. Dr. Muhammad Javaid Asad is a Ph.D and Post Doc has worked as associate professor.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2011
Langue
anglais
ISBN 10 
3846509264
ISBN 13 
9783846509265
Reliure
Broché
Nombre de pages
140
Coordonnées du fabricant
non disponible
Personne responsable
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