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Methodology for Extraction and Purification of ISOMERASE: Polarimetric Determination of Mutarotase; an Isomerase - Couverture souple

Siddique, Amna; Khurshid, Shazia

 
9783846522530: Methodology for Extraction and Purification of ISOMERASE: Polarimetric Determination of Mutarotase; an Isomerase
Couverture souple
ISBN 10 : 3846522538 ISBN 13 : 9783846522530
Editeur : LAP LAMBERT Academic Publishing, 2011

Synopsis

Mutarotases are recently characterized group of enzymes that are involved in anomeric conversions of monosaccharides enhancing rate of metabolism. Mutarotase was extracted and purified from bovine kidney cortex by (NH4)2SO4 precipitation technique, dialysis and gel filtration chromatography. Polarimeter was used for assay of Mutarotase in this present project. Mutarotase increased activity from 0.478 to 1.711 U/ml by purification. It gained 13.4 folds purification at this final step. It is helpful in determining the glucose level and participates in glycolysis and gluconeogenesis. Activities and fold purification of Mutarotase can also be increased by much improved techniques of purification like ion exchange chromatography or FPLC hence enhancing the production of this enzyme.

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Présentation de l'éditeur

Mutarotases are recently characterized group of enzymes that are involved in anomeric conversions of monosaccharides enhancing rate of metabolism. Mutarotase was extracted and purified from bovine kidney cortex by (NH4)2SO4 precipitation technique, dialysis and gel filtration chromatography. Polarimeter was used for assay of Mutarotase in this present project. Mutarotase increased activity from 0.478 to 1.711 U/ml by purification. It gained 13.4 folds purification at this final step. It is helpful in determining the glucose level and participates in glycolysis and gluconeogenesis. Activities and fold purification of Mutarotase can also be increased by much improved techniques of purification like ion exchange chromatography or FPLC hence enhancing the production of this enzyme.

Biographie de l'auteur

Amna Siddique: M.Phil Chemistry, GC University Lahore, Pakistan worked on Production of Enzymes and their Purification.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2011
Langue
anglais
ISBN 10 
3846522538
ISBN 13 
9783846522530
Reliure
Broché
Nombre de pages
72
Coordonnées du fabricant
non disponible
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