Articles liés à Fungal pathogen in microbial keratitis: Evaluation...

Fungal pathogen in microbial keratitis: Evaluation of PCR method to detect fungal pathogen in microbial keratitis - Couverture souple

Embong, Zunaina; Wan Hitam, Wan Hazabbah; Ravichandran, Manickam

 
9783846532249: Fungal pathogen in microbial keratitis: Evaluation of PCR method to detect fungal pathogen in microbial keratitis
Couverture souple
ISBN 10 : 384653224X ISBN 13 : 9783846532249
Editeur : LAP LAMBERT Academic Publishing, 2011

Synopsis

Microbial keratitis is a serious ocular infection. It is often difficult to establish a microbial keratitis that is due to fungal infection. Inadequate therapy and delayed diagnosis of fungal keratitis leads to generally poor visual outcomes. The objective of this study is to determine the sensitivity and specificity of PCR to detect fungal pathogen in microbial keratitis. In this study, a PCR method was compared to the conventional method to detect fungal pathogen in microbial keratitis. The PCR was performed with primers pair F1,R2 and F3,R4 from two consecutive regions in the 18S rRNA gene. Positive result for PCR was considered when there was presence of this amplified target sequence in both primer pairs F1,R2 and F3,R4. A statistical diagnostic test revealed that the PCR has a sensitivity of 90.9% and a specificity of 94.7% to detect fungal pathogen in microbial keratitis. PCR is a rapid, sensitive and a useful method to detect fungal pathogen in microbial keratitis.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Microbial keratitis is a serious ocular infection. It is often difficult to establish a microbial keratitis that is due to fungal infection. Inadequate therapy and delayed diagnosis of fungal keratitis leads to generally poor visual outcomes. The objective of this study is to determine the sensitivity and specificity of PCR to detect fungal pathogen in microbial keratitis. In this study, a PCR method was compared to the conventional method to detect fungal pathogen in microbial keratitis. The PCR was performed with primers pair F1,R2 and F3,R4 from two consecutive regions in the 18S rRNA gene. Positive result for PCR was considered when there was presence of this amplified target sequence in both primer pairs F1,R2 and F3,R4. A statistical diagnostic test revealed that the PCR has a sensitivity of 90.9% and a specificity of 94.7% to detect fungal pathogen in microbial keratitis. PCR is a rapid, sensitive and a useful method to detect fungal pathogen in microbial keratitis.

Biographie de l'auteur

Dr Zunaina Embong is a Medical Lecturer and Consultant Ophthalmologist in the Department of Ophthalmology, Universiti Sains Malaysia, Malaysia.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2011
Langue
anglais
ISBN 10 
384653224X
ISBN 13 
9783846532249
Reliure
Broché
Nombre de pages
88
Coordonnées du fabricant
non disponible
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