Mapping of RAPD primers on chromosome 2A of common wheat: Mapping of chromosome 2A of common wheat triticum aestivum - Couverture souple

Parveen, Khamsa; Ahmad, Israr

 
9783846550199: Mapping of RAPD primers on chromosome 2A of common wheat: Mapping of chromosome 2A of common wheat triticum aestivum
Couverture souple
ISBN 10 : 3846550191 ISBN 13 : 9783846550199
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

Common (bread) wheat is an allohexaploid species (Triticum aestivum L.) with 3 compensating genomes AABBDD (2n=6x=42 chromosomes). Traditionally genetic and physical mapping in this most important cereal crop has been based on molecular markers (RFLPs, PCR, Insitu hubridisation etc). The recent development in producing deletion lines of common wheat (using Aegilops cylindricum genome) has revolutionized the physical mapping of wheat chromosomes. During present study, deletion lines of chromosomes 2A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 2A. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 2A.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Common (bread) wheat is an allohexaploid species (Triticum aestivum L.) with 3 compensating genomes AABBDD (2n=6x=42 chromosomes). Traditionally genetic and physical mapping in this most important cereal crop has been based on molecular markers (RFLPs, PCR, Insitu hubridisation etc). The recent development in producing deletion lines of common wheat (using Aegilops cylindricum genome) has revolutionized the physical mapping of wheat chromosomes. During present study, deletion lines of chromosomes 2A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 2A. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 2A.

Biographie de l'auteur

I Miss Khamsa Parveen did my Master in Genetics from Hazara University Mansehra Pakistan. My research topic was “MAPPING OF RANDOMLY AMPLIFIED POLYMORPHIC DNA PRIMERS (RAPD) ON CHROMOSOME 2A OF COMMON WHEAT TRITICUM AESTIVUM (L). I did my research work in Functional Genomic Lab (FGL)of the said department.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3846550191
ISBN 13 
9783846550199
Reliure
Broché
Nombre de pages
68
Coordonnées du fabricant
non disponible
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