Characterization of 3-Hydroxy-3-Methylglutaryl Coenzyme a Reductase - Couverture souple

Samreen Khush Bakhat ; Saleem Yasar

9783846554173: Characterization of 3-Hydroxy-3-Methylglutaryl Coenzyme a Reductase

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ISBN 10 : 3846554170 ISBN 13 : 9783846554173

Editeur : LAP Lambert Academic Publishing, 2014

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�diteur: LAP Lambert Academic Publishing
Date d'�dition: 2014
Langue: anglais
ISBN 10: 3846554170
ISBN 13: 9783846554173
Reliure: Broch�
Nombre de pages: 104
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Characterization Of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase

Khush Bakhat Samreen

Edit� par LAP LAMBERT Academic Publishing Feb 2014, 2014

ISBN 10 : 3846554170 ISBN 13 : 9783846554173

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonate, which is known as the rate-limiting step in the biosynthesis of cholesterol and other intermediates of mevalonate pathway.The purified plasmid (pGEXtev/hmgr) containing gene of human HMG-CoA reductase was confirmed by restriction analysis and sequencing. After verification, it was transformed into competent cells of Escherchia coli (BL21 and DH5 ) for which DH5 produced most promising results. Protein expression was induced with IPTG and further optimized through varying the concentration of IPTG, betain, sorbitol and addition of various buffers. The influence of various temperatures on protein expression was also investigated. The gene of interest (hmgr) was sub cloned into pET 22b. Sub cloned plasmid pET22b/hmgr was induced in E. coli BL21and its expression was optimized by employing different IPTG concentrations and taking samples at different ODs (A600). The plasmid pGEXtev/hmgr in DH5 expresses a protein of 54 kDa along with GST fusion tag. The protein consists of 283 amino acids and had a molecular weight of 31.1 kDa and theoretical isoelectric point is 7.68. 104 pp. Englisch. N� de r�f. du vendeur 9783846554173

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Characterization of 3-Hydroxy-3-Methylglutaryl Coenzyme a Reductase

Samreen Khush Bakhat; Saleem Yasar

Edit� par VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2014

ISBN 10 : 3846554170 ISBN 13 : 9783846554173

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Etat : New. pp. 104. N� de r�f. du vendeur 26127773107

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Characterization Of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase

Khush Bakhat Samreen|Yasar Saleem|Faiza Saleem

Edit� par LAP LAMBERT Academic Publishing, 2014

ISBN 10 : 3846554170 ISBN 13 : 9783846554173

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Samreen Khush BakhatKhush Bakhat Samreen had done her M-phill in Biotechnology from Lahore College for Women Univrsity, Lahore, Pakistan. She has publications in the form of book and paper. Her specific area of research was Molecular. N� de r�f. du vendeur 5498675

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Characterization of 3-Hydroxy-3-Methylglutaryl Coenzyme a Reductase

Samreen Khush Bakhat; Saleem Yasar

Edit� par VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2014

ISBN 10 : 3846554170 ISBN 13 : 9783846554173

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Etat : New. Print on Demand pp. 104 2:B&W 6 x 9 in or 229 x 152 mm Perfect Bound on Creme w/Gloss Lam. N� de r�f. du vendeur 132781676

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Characterization of 3-Hydroxy-3-Methylglutaryl Coenzyme a Reductase

Samreen Khush Bakhat; Saleem Yasar

Edit� par VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2014

ISBN 10 : 3846554170 ISBN 13 : 9783846554173

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Etat : New. PRINT ON DEMAND pp. 104. N� de r�f. du vendeur 18127773113

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Characterization Of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase

Khush Bakhat Samreen

Edit� par LAP LAMBERT Academic Publishing Feb 2014, 2014

ISBN 10 : 3846554170 ISBN 13 : 9783846554173

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonate, which is known as the rate-limiting step in the biosynthesis of cholesterol and other intermediates of mevalonate pathway.The purified plasmid (pGEXtev/hmgr) containing gene of human HMG-CoA reductase was confirmed by restriction analysis and sequencing. After verification, it was transformed into competent cells of Escherchia coli (BL21 and DH5�) for which DH5� produced most promising results. Protein expression was induced with IPTG and further optimized through varying the concentration of IPTG, betain, sorbitol and addition of various buffers. The influence of various temperatures on protein expression was also investigated. The gene of interest (hmgr) was sub cloned into pET 22b. Sub cloned plasmid pET22b/hmgr was induced in E. coli BL21and its expression was optimized by employing different IPTG concentrations and taking samples at different ODs (A600). The plasmid pGEXtev/hmgr in DH5� expresses a protein of 54 kDa along with GST fusion tag. The protein consists of 283 amino acids and had a molecular weight of 31.1 kDa and theoretical isoelectric point is 7.68.VDM Verlag, Dudweiler Landstra�e 99, 66123 Saarbr�cken 104 pp. Englisch. N� de r�f. du vendeur 9783846554173

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Characterization Of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase : Human HMG-CoA Reductase Protein Expression

Faiza Saleem

Edit� par LAP LAMBERT Academic Publishing, 2014

ISBN 10 : 3846554170 ISBN 13 : 9783846554173

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Vendeur : AHA-BUCH GmbH, Einbeck, Allemagne

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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonate, which is known as the rate-limiting step in the biosynthesis of cholesterol and other intermediates of mevalonate pathway.The purified plasmid (pGEXtev/hmgr) containing gene of human HMG-CoA reductase was confirmed by restriction analysis and sequencing. After verification, it was transformed into competent cells of Escherchia coli (BL21 and DH5 ) for which DH5 produced most promising results. Protein expression was induced with IPTG and further optimized through varying the concentration of IPTG, betain, sorbitol and addition of various buffers. The influence of various temperatures on protein expression was also investigated. The gene of interest (hmgr) was sub cloned into pET 22b. Sub cloned plasmid pET22b/hmgr was induced in E. coli BL21and its expression was optimized by employing different IPTG concentrations and taking samples at different ODs (A600). The plasmid pGEXtev/hmgr in DH5 expresses a protein of 54 kDa along with GST fusion tag. The protein consists of 283 amino acids and had a molecular weight of 31.1 kDa and theoretical isoelectric point is 7.68. N� de r�f. du vendeur 9783846554173

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Characterization Of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase | Human HMG-CoA Reductase Protein Expression

Khush Bakhat Samreen (u. a.)

Edit� par LAP LAMBERT Academic Publishing, 2014

ISBN 10 : 3846554170 ISBN 13 : 9783846554173

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