In vitro Regeneration and Genetic Transformation of Ricinus communis: An Approach towards Castor Improvement - Couverture souple

Kansara, Rohan V.; Jha, Sanjay; Jha, Suman Kumar

Couverture souple

ISBN 10 : 3847374265 ISBN 13 : 9783847374268

Editeur : LAP LAMBERT Academic Publishing, 2012

A propos du livre

Présentation de l'éditeur

An efficient in vitro plant regeneration and Agrobacterium tumefaciens mediated genetic transformation protocol was described for castor (Ricinus communis L.) using meristem as explants. Shoot apex containing apical meristems were excised from 5-7 days old in vitro grown seedlings and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins singly or in combination. Kinetin (0.50 mg/l) in combination with BAP (0.25 mg/l) produced maximum number of shoot (10.33) and shoot length (5.20 cm). For root induction, in vitro produced shoots were transferred to rooting media containing ½ MS basal media with NAA. In Agrobacterium tumefaciens mediated genetic transformation, Agrobacterium tumefaciens strain containing construct pBIN1F harboring nptII and cry1F gene was used. The integration of the gene was confirmed by using PCR. The transformation experiment was performed by optimizing age of the seedlings and co-cultivation duration with Agrobacterium. When the effect of the age of the seedlings and co-cultivation duration evaluated fifteen days old seedlings and co-cultivated for three days yielded the frequency of transformation 2%.

Biographie de l'auteur

Rohan V. Kansara is a Biotechnologist holding a Post graduate degree in Plant Biotechnology from Navsari Agricultural University, Gujarat, India. He is currently working as Project assistant on heavy metal tolerance and transgenic in castor and rice in the same university. The publications of the author are accepted in peer reviewed journal.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2012
• Langue: anglais
• ISBN 10: 3847374265
• ISBN 13: 9783847374268
• Reliure: Broché
• Nombre de pages: 128
• Coordonnées du fabricant: non disponible
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