Development of Molecular Markers for chromosome 5A of common wheat: Development of RAPD-Based Molecular Markers for Chromosome 5A of Common Whear - Couverture souple

Ali, Amjad; Nisar, Mohammad; Hussain, Shah

Couverture souple

ISBN 10 : 3848406217 ISBN 13 : 9783848406210

Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

Common (bread) wheat is an allohexaploid species (Triticum aestivum L.) with 3 compensating genomes AABBDD (2n=6x=42 chromosomes). Traditionally genetic and physical mapping in this most important cereal crop has been based on molecular markers (RFLPs, PCR, Insitu hybridization etc). The recent development in producing deletion lines of common wheat (using Aegilops cylindricum genome) has revolutionized the physical mapping of wheat chromosomes. During present study, deletion lines of chromosomes 5A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 5A. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism for long arm deletion lines viz; del5AL-10 and del5AL-23. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 5A.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2012
• Langue: anglais
• ISBN 10: 3848406217
• ISBN 13: 9783848406210
• Reliure: Broché
• Nombre de pages: 56
• Coordonnées du fabricant: non disponible
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