Somatic Cell Nuclear Transfer in Goat (Capra hircus): Production of Cloned Embryos by Hand-made Cloning Method - Couverture souple

Akshey, Yogesh S.; De, Arun Kumar; Malakar, Dhruba

 
9783848426546: Somatic Cell Nuclear Transfer in Goat (Capra hircus): Production of Cloned Embryos by Hand-made Cloning Method
Couverture souple
ISBN 10 : 3848426544 ISBN 13 : 9783848426546
Editeur : LAP Lambert Academic Publishing, 2012

Synopsis

Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

À propos de l?auteur

Yogesh S. Akshey, Arun Kumar De and Dhruba Malakar:Currently working on in vitro and in vivo production of embryos and their transfer to recipient animals, stem cells, somatic cell nuclear transfer, induced pluripotent stem cells and regulation of oocyte competence in farm animals. Published a number of research articles in reputed journals.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP Lambert Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3848426544
ISBN 13 
9783848426546
Reliure
Broché
Nombre de pages
128
Coordonnées du fabricant
non disponible
Personne responsable
KreGa Onlinehandel GmbH
4950000000000
info@krega-onlinehandel.de

Birkenweg 31
Brakel
33034
Allemagne