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Testing promoter trapping activity of a new vector (pNU435) in tomato: Promoter trapping - Couverture souple

Biradar, S. S.

 
9783848429844: Testing promoter trapping activity of a new vector (pNU435) in tomato: Promoter trapping
Couverture souple
ISBN 10 : 3848429845 ISBN 13 : 9783848429844
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

This study aimed at transforming PUSA-RUBY, a cultivar of tomato with a new promoter trapping vector pNU435 using Agrobacterium-mediated transformation. The Agrobacterium strain LBA4404 carrying pNU435 when co-cultivated with tomato leaves, sixteen plantlets (T1) were obtained of which 6 were found to be PCR positive and among them two PCR positive plants (PT4 and PT5) upon RB TAIL-PCR, produced amplicon of ~400bp which were cloned into pTZ57R/T by T/A cloning and sequence of the RB TAIL-PCR product with M13 F/R primers upon BLAST search showed homology to right border of T-DNA. This study demands the need for generating and testing large number of independent transgenic events for promoter trapping. At the same time, these lines can be tested for promoter trapping under special conditions such as biotic and abiotic stresses.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

This study aimed at transforming PUSA-RUBY, a cultivar of tomato with a new promoter trapping vector pNU435 using Agrobacterium-mediated transformation. The Agrobacterium strain LBA4404 carrying pNU435 when co-cultivated with tomato leaves, sixteen plantlets (T1) were obtained of which 6 were found to be PCR positive and among them two PCR positive plants (PT4 and PT5) upon RB TAIL-PCR, produced amplicon of ~400bp which were cloned into pTZ57R/T by T/A cloning and sequence of the RB TAIL-PCR product with M13 F/R primers upon BLAST search showed homology to right border of T-DNA. This study demands the need for generating and testing large number of independent transgenic events for promoter trapping. At the same time, these lines can be tested for promoter trapping under special conditions such as biotic and abiotic stresses.

Biographie de l'auteur

Siddanagouda S Biradar is an PhD student at North-West A & F University, Yangling, China. He is from Padekanur, small village of Bijapur district, muddebihal taluk, Karnataka India. He completed his B.Sc(Agri.) and M.Sc Agri(Plant Biotechnology) from UAS Dharwad. He is an gold medalist at UG and meritorious student throughout his career.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3848429845
ISBN 13 
9783848429844
Reliure
Broché
Nombre de pages
60
Coordonnées du fabricant
non disponible
Personne responsable
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