ELISA - Enzyme Linked Immunosorbent Assay: reliable and cheap method for the detection of food allergens - Couverture souple

Iqbal, Amjad

 
9783848436637: ELISA - Enzyme Linked Immunosorbent Assay: reliable and cheap method for the detection of food allergens
Couverture souple
ISBN 10 : 3848436639 ISBN 13 : 9783848436637
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

In ELISA formats there are several parameters that are common and critical to assay performance and should be considered during the optimization process, including antibodies concentration, antigen concentration, incubation time of blocking, substrate solution and washing solution. Though immunoassays offer a specific, sensitive and rapid method to detect and quantify even trace amounts of allergens in foodstuffs, certain processes can denature, alter or destroy proteins so that they are no longer detectible by assay using antibodies. When using monoclonal antibodies, there is a great chance of obtaining false negatives in the detection. Polyclonal antibodies are recommended for ELISA techniques as the risk of obtaining false negatives is reduced as compared to monoclonal antibodies which are quite specific.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

In ELISA formats there are several parameters that are common and critical to assay performance and should be considered during the optimization process, including antibodies concentration, antigen concentration, incubation time of blocking, substrate solution and washing solution. Though immunoassays offer a specific, sensitive and rapid method to detect and quantify even trace amounts of allergens in foodstuffs, certain processes can denature, alter or destroy proteins so that they are no longer detectible by assay using antibodies. When using monoclonal antibodies, there is a great chance of obtaining false negatives in the detection. Polyclonal antibodies are recommended for ELISA techniques as the risk of obtaining false negatives is reduced as compared to monoclonal antibodies which are quite specific.

Biographie de l'auteur

Dr. Amjad Iqbal has obtained his Masters degree in Food Science and Technology from University of Ghent and Katholic University of Belgium in 2005. He then earned his PhD and Post-Doc from The University of Edinburgh 2006-2010. He is currently working for Scottish Government and have published articles in peer reviewed journals.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3848436639
ISBN 13 
9783848436637
Reliure
Broché
Nombre de pages
80
Coordonnées du fabricant
non disponible
Personne responsable
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