Human skeletal remains:development of DNA extraction and typing assays: DNA extraction and typing of old human bones - Couverture souple

Filippini, Giulia; Turrina, Stefania; De Leo, Domenico

 
9783848436972: Human skeletal remains:development of DNA extraction and typing assays: DNA extraction and typing of old human bones
Couverture souple
ISBN 10 : 3848436973 ISBN 13 : 9783848436972
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

Efficient DNA extraction procedures, as well as accurate DNA amplification, are critical steps involved in the process of successful DNA analysis of skeletal samples. Unfortunately, at present there is not an infallible method to recover DNA from very degraded samples due to variations in DNA yield from bone fragments that may be attributed to heterogeneity within a bones. In this study different types of human bones ranging in age from few months to 90 post mortem years, found in various states of preservation and conserved in different places, were analyzed. We developed a modified silica based spin columns protocol, associated to the typing of conventional Short Tandem Repeats (STRs) and smaller Polymerase Chain Reaction (PCR) products (Mini-STRs) redesigned by our laboratory by moving forward and reverse primers in close proximity to the STR repeat region. Our modified protocol was successfully employed to extract DNA from long bones of different ages and preservation state. Importantly the use of phenol chloroform consistently increased the amount of DNA that could be extracted from long bones, preventing that waste material interferes with columns or magnetic beads.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Efficient DNA extraction procedures, as well as accurate DNA amplification, are critical steps involved in the process of successful DNA analysis of skeletal samples. Unfortunately, at present there is not an infallible method to recover DNA from very degraded samples due to variations in DNA yield from bone fragments that may be attributed to heterogeneity within a bones. In this study different types of human bones ranging in age from few months to 90 post mortem years, found in various states of preservation and conserved in different places, were analyzed. We developed a modified silica based spin columns protocol, associated to the typing of conventional Short Tandem Repeats (STRs) and smaller Polymerase Chain Reaction (PCR) products (Mini-STRs) redesigned by our laboratory by moving forward and reverse primers in close proximity to the STR repeat region. Our modified protocol was successfully employed to extract DNA from long bones of different ages and preservation state. Importantly the use of phenol chloroform consistently increased the amount of DNA that could be extracted from long bones, preventing that waste material interferes with columns or magnetic beads.

Biographie de l'auteur

Giulia Filippini has obtained her Master degree in Sanitary Biology at University of Padua and she got PhD in Forensic Medicine and Science at University of Verona in 2010. She has acquired of experience in Forensic Genetic field, producing more than ten peer- reviewed articles. Giulia is carrying on with her studies as post-doctoral researcher.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3848436973
ISBN 13 
9783848436972
Reliure
Broché
Nombre de pages
80
Coordonnées du fabricant
non disponible
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