Basic Molecular Biology Techniques: Protocol for Various PCR Varities - Couverture souple

Kumar, Amar Nagesh; Kumar, Robby

Couverture souple

ISBN 10 : 3848442345 ISBN 13 : 9783848442348

Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

A primer is a strand of nucleic acid that serves as a starting point for DNA replication. They are required because the enzymes that catalyze replication, DNA polymerases, can only add new nucleotides to an existing strand of DNA.The primers are to be designed correctly in thr amplification of a single DNA fragment corresponding to the target reagion of the template molecule.The primer should be complementary to its template strand at the 5' and 3' ends of the DNA region. in order for hybridization to occur . Amplification can occur when mismatching primers are close enough together on opposite strands of DNA - and an unwanted sequence is produced with ends that precisely match the primers. If such an 'incorrect' fragment is synthesized in the early cycles of a PCR, it will be efficiently amplified on subsequent cycles.

A propos du livre

À propos de l'auteur

I am an Academician cum Researcher in Biochemistry for the past three years. Till now I have published 7 papers (Including research and review) in international peer reviewed journals.Currently working as Sr Lecturer in Biochemistry Dept. of SSR Medical College, Mauritius since one year.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2012
• Langue: anglais
• ISBN 10: 3848442345
• ISBN 13: 9783848442348
• Reliure: Broché
• Nombre de pages: 100
• Coordonnées du fabricant: non disponible
• Personne responsable: KreGa Onlinehandel GmbH; 4950000000000; info@krega-onlinehandel.de; ; Birkenweg 31; Brakel; 33034; Allemagne