Articles liés à Isolation And Amplification Of Β-Lactamage Gene...

Isolation And Amplification Of Β-Lactamage Gene From S.Aureus: Gene And Staphylococcus Aureus - Couverture souple

Thayyuru, Geetharathan

 
9783848480784: Isolation And Amplification Of Β-Lactamage Gene From S.Aureus: Gene And Staphylococcus Aureus
Couverture souple
ISBN 10 : 3848480786 ISBN 13 : 9783848480784
Editeur : LAP LAMBERT Academic Publishing, 2018

Synopsis

Clinical isolate of S.aureus showing resistance to ampicillin was chosen in the present study for isolation and amplification of ß-lactamase gene was successfully amplified from chromosomal DNA using the primers constructed from class (A) ß-lactamase gene of M.tuberculosis H37Rv. PCR product was cloned into the SmaI site of M13 mp18 vector and positive clone was selected using 100µg/ml ampicillin and further, the DNA from the clone was isolated and checked for the insert using the same primers. PCR product (0.7Kb) indicated that the cloning was successful which also confirmed that ß-lactamase that confers resistance to ampicillin and to other ß-lactam antibiotics was present on the chromosomal DNA of S.aurues.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2018
Langue
anglais
ISBN 10 
3848480786
ISBN 13 
9783848480784
Reliure
Broché
Nombre de pages
52
Coordonnées du fabricant
non disponible
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