Atomic Force Microscopy applied to biological systems: Study of Xenopus laevis oocyte plasma membrane - Couverture souple

Orsini, Francesco

 
9783848493944: Atomic Force Microscopy applied to biological systems: Study of Xenopus laevis oocyte plasma membrane
Couverture souple
ISBN 10 : 3848493942 ISBN 13 : 9783848493944
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

This book describes the application of Atomic Force Microscopy (AFM) to the study of the Xenopus laevis oocyte plasma membrane. In particular, different sample preparation protocols developed and optimized to perform AFM investigation of both external and intracellular sides of the oocyte native plasma membrane are presented and discussed in details. AFM imaging allowed visualization and dimensional characterization of protein complexes observed on both sides of the oocyte plasma membrane. In addition, a new methodological approach based on the purification of oocyte plasma membrane by ultracentrifugation on a discontinuous sucrose gradient allowed to image oocyte membrane in a physiological-like environment as well as to identify a membrane protein, the human aquaporin 4, expressed in the oocytes thus opening interesting perspectives in the AFM investigation of heterologous proteins of relevant biomedical interest. Taken together, these results confirm the potential of AFM as a useful tool for the structural characterization of proteins in native eukaryotic membranes as well as its relevance for describing the organization of protein complexes in native biological membranes.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3848493942
ISBN 13 
9783848493944
Reliure
Broché
Nombre de pages
216
Coordonnées du fabricant
non disponible
Personne responsable
KreGa Onlinehandel GmbH
4950000000000
info@krega-onlinehandel.de

Birkenweg 31
Brakel
33034
Allemagne