Synopsis
The techniques for establishing and maintaining invertebrate tissues and cells in culture remain difficult due to the diversity of invertebrates and their structural and physiological characteristics. Research involving invertebrate cell cultures continues to increase, although the number of cell lines used is still limited. This manual gives detailed descriptions of the technical procedures for the establishment of primary invertebrate cell cultures in vitro. Nutritional requirements, culture media, and species-specific methods for both cell and organ cultures as well as useful techniques for studies on cultured cells are described. The Appendix lists established cell lines available for research with information on the composition of their physiological and nutrient solutions. This comprehensive manual, the first of its kind, is a valuable reference for investigators working with invertebrate cell cultures in academia and industry.
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Invertebrate tissue culture methods.
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Tokyo, Springer., 2002
ISBN 10 : 4431703136
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XVI, 446 p. Softcover. Versand aus Deutschland / We dispatch from Germany via Air Mail. Einband bestoßen, daher Mängelexemplar gestempelt, sonst sehr guter Zustand. Imperfect copy due to slightly bumped cover, apart from this in very good condition. Stamped. Stamped. Springer lab manual. Sprache: Englisch. N° de réf. du vendeur 39358HB
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Invertebrate tissue culture methods. Springer lab manual
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Broschiert. Etat : Gut. 446 Seiten; Der Erhaltungszustand des hier angebotenen Werks ist trotz seiner Bibliotheksnutzung sehr sauber. Es befindet sich neben dem Rückenschild lediglich ein Bibliotheksstempel im Buch; ordnungsgemäß entwidmet. In ENGLISCHER Sprache. Sprache: Englisch Gewicht in Gramm: 750. N° de réf. du vendeur 2013324
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Invertebrate Tissue Culture Methods (Springer Lab Manuals)
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Invertebrate Tissue Culture Methods
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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials How could I remove tissues from a tiny insect How many tissues should I pool in order to set up one culture I had to find out the answers. Naturally, it took a lot of time. 464 pp. Englisch. N° de réf. du vendeur 9784431703136
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. With step-by-step protocols, it introduces methods to initiate tissue culture from invertebrates including, Polifera, Coelenterata, Plathelminthes, Nematoda, Annedlia, Mollusca, Echinodermata, and ProchordataThe Appendix lists established cell lines ava. N° de réf. du vendeur 5753579
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Invertebrate Tissue Culture Methods
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Taschenbuch. Etat : Neu. Invertebrate Tissue Culture Methods | Jun Mitsuhashi | Taschenbuch | xvi | Englisch | 2002 | Springer | EAN 9784431703136 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. N° de réf. du vendeur 104203790
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Springer Japan, Springer Japan Feb 2002, 2002
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ISBN 13 : 9784431703136
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Taschenbuch. Etat : Neu. Neuware -I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials How could I remove tissues from a tiny insect How many tissues should I pool in order to set up one culture I had to find out the answers. Naturally, it took a lot of time.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 464 pp. Englisch. N° de réf. du vendeur 9784431703136
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