Morphological and Biochemical Characterization of Cellulolytic enzyme - Couverture souple

Singh, Pushpanjali; Sarkar, Ankita; Srivashtav, Vishal

Couverture souple

ISBN 10 : 6200117470 ISBN 13 : 9786200117472

Editeur : LAP LAMBERT Academic Publishing, 2019

Synopsis

Cellulases are enzyme complex of hydrolytic enzymes such as endoglucanase (CMCase), exoglucanase, β-glucosidase (BGL) and FPase that are responsible for the bioconversion of the cellulosic biomass into valuable products. Aspergillus niger were isolated from the waste of rice field and grown on potato dextrose agar medium. Morphological studies were done on different pH, incubation period and different concentration of ammonium sulphate. pH 7.7, 72 hrs of incubation and 0.14% ammonium sulphate were most suitable for the growth of Aspergillus niger. Plate screening was done by using carboxy methyl cellulose as carbon source which showed clear zone formation on staining with congo red dye. By using DNSA method, cellulase activity was observed at different nitrogen source with different concentration, pH and incubation time. pH 4.2, 96 hrs incubation period, 0.125% peptone, 0.14% ammoniumsulphate and 0.04% urea were best for cellulase activity.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2019
• Langue: anglais
• ISBN 10: 6200117470
• ISBN 13: 9786200117472
• Reliure: Broché
• Nombre de pages: 92
• Coordonnées du fabricant: non disponible
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