Interferon LAMBDA-3: Immune Modulator: Role of pcDNA-Interferon LAMBDA-3 (pcDNA-IFNL3) as Immune Modulator - Couverture souple

S, Barathiraja; G.R, Reddy

 
9786200311818: Interferon LAMBDA-3: Immune Modulator: Role of pcDNA-Interferon LAMBDA-3 (pcDNA-IFNL3) as Immune Modulator
Couverture souple
ISBN 10 : 6200311811 ISBN 13 : 9786200311818
Editeur : LAP LAMBERT Academic Publishing, 2019

Synopsis

Interferon Lambdas (IFNLs) is a type III IFN which induces expression of Interferon Stimulated Genes (ISGs). These ISGs in turn participate in antiviral and other interferon related activities. IFNLs are found to have immune modulation activities in addition to antiviral activities. Immune modulation activities are advantageous for the host system for better protection against invading pathogens. In the present study, the vector construct of bovine Interferon lambda-3 (IFN-L3) was analyzed for its immune modulation activities. The IFNL3 gene of 585bp cloned in pcDNA3.1+ vector (pcDNA-IFNL3) was analyzed for its expression in baby hamster kidney (BHK) cell line by fluorescence microscopy. Bovine IFNL3 gene cloned in pcDNA3.1+ vector with downstream Green fluorescent protein (GFP) tag found to show the green fluorescence. This construct was further tested for its immune modulation activities in mice. At 7th day the Spleenocytes were collected from the mice and analyzed for activation of B and T lymphocytes by Fluorescent Activated Cell Sorting (FACS) using specific CD markers. The results showed that IFNλ3 could activate both B and T lymphocyte population in treated mice.

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Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2019
Langue
anglais
ISBN 10 
6200311811
ISBN 13 
9786200311818
Reliure
Broché
Nombre de pages
56
Coordonnées du fabricant
non disponible
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