NGS Approach for Variant Discovery in Giant Freshwater Prawn: An NGS approach to mine SNP from the amplicons of Macrobrachium rosenbergii - Couverture souple

Agarwal, Deepak ; Chaudhari, Aparna

9786200440051: NGS Approach for Variant Discovery in Giant Freshwater Prawn: An NGS approach to mine SNP from the amplicons of Macrobrachium rosenbergii

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ISBN 10 : 6200440050 ISBN 13 : 9786200440051

Editeur : LAP LAMBERT Academic Publishing, 2019

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Amongst farmed crustaceans, the giant freshwater prawn Macrobrachium rosenbergii was until recently one of the major species with high commercial value. Lately, it has lost popularity due to diseases and differential growth of males on account of social hierarchy. The species can reacquire its former preferred status if it is genetically improved for growth and disease resistance. Selective breeding is greatly facilitated by marker-assisted selection (MAS). Single nucleotide polymorphisms (SNPs) offer the finest resolution possible for a marker map (a single nucleotide change), are co-dominant, bi-allelic and generally abundant in populations with a low mutation rate. DNA sequencing through NGS platforms is regarded as the most cost-effective approach. In this study, SNP mining was carried out in the transcribed sequences of M. rosenbergii available in GenBank, NCBI. Wild specimens were collected from different water bodies. Primers were designed to amplify coding (CDS) and sequenced the amplicons on GS-FLX 454 and Ion torrent platforms. 173 SNPs were detected across populations. This approach for mining SNPs in reported sequences has been used by us for the first time.

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�diteur: LAP LAMBERT Academic Publishing
Date d'�dition: 2019
Langue: anglais
ISBN 10: 6200440050
ISBN 13: 9786200440051
Reliure: Broch�
Nombre de pages: 88
Coordonn�es du fabricant: non disponible
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NGS Approach for Variant Discovery in Giant Freshwater Prawn

Deepak Agarwal

Edit� par LAP LAMBERT Academic Publishing Okt 2019, 2019

ISBN 10 : 6200440050 ISBN 13 : 9786200440051

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Amongst farmed crustaceans, the giant freshwater prawn Macrobrachium rosenbergii was until recently one of the major species with high commercial value. Lately, it has lost popularity due to diseases and differential growth of males on account of social hierarchy. The species can reacquire its former preferred status if it is genetically improved for growth and disease resistance. Selective breeding is greatly facilitated by marker-assisted selection (MAS). Single nucleotide polymorphisms (SNPs) offer the finest resolution possible for a marker map (a single nucleotide change), are co-dominant, bi-allelic and generally abundant in populations with a low mutation rate. DNA sequencing through NGS platforms is regarded as the most cost-effective approach. In this study, SNP mining was carried out in the transcribed sequences of M. rosenbergii available in GenBank, NCBI. Wild specimens were collected from different water bodies. Primers were designed to amplify coding (CDS) and sequenced the amplicons on GS-FLX 454 and Ion torrent platforms. 173 SNPs were detected across populations. This approach for mining SNPs in reported sequences has been used by us for the first time. 88 pp. Englisch. N� de r�f. du vendeur 9786200440051

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NGS Approach for Variant Discovery in Giant Freshwater Prawn

Deepak agarwal|Aparna Chaudhari

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ISBN 10 : 6200440050 ISBN 13 : 9786200440051

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: agarwal DeepakDeepak Agarwal has done graduation in fisheries science from G.B.P.U.A &amp T Pantnagar University. He holds his Ph.D. in Fish Biotechnology from CIFE, Mumbai. He received a VC gold medal for the highest marks in UG an. N� de r�f. du vendeur 330554007

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NGS Approach for Variant Discovery in Giant Freshwater Prawn

Deepak Agarwal

Edit� par LAP LAMBERT Academic Publishing Okt 2019, 2019

ISBN 10 : 6200440050 ISBN 13 : 9786200440051

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -Amongst farmed crustaceans, the giant freshwater prawn Macrobrachium rosenbergii was until recently one of the major species with high commercial value. Lately, it has lost popularity due to diseases and differential growth of males on account of social hierarchy. The species can reacquire its former preferred status if it is genetically improved for growth and disease resistance. Selective breeding is greatly facilitated by marker-assisted selection (MAS). Single nucleotide polymorphisms (SNPs) offer the finest resolution possible for a marker map (a single nucleotide change), are co-dominant, bi-allelic and generally abundant in populations with a low mutation rate. DNA sequencing through NGS platforms is regarded as the most cost-effective approach. In this study, SNP mining was carried out in the transcribed sequences of M. rosenbergii available in GenBank, NCBI. Wild specimens were collected from different water bodies. Primers were designed to amplify coding (CDS) and sequenced the amplicons on GS-FLX 454 and Ion torrent platforms. 173 SNPs were detected across populations. This approach for mining SNPs in reported sequences has been used by us for the first time.VDM Verlag, Dudweiler Landstra�e 99, 66123 Saarbr�cken 88 pp. Englisch. N� de r�f. du vendeur 9786200440051

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NGS Approach for Variant Discovery in Giant Freshwater Prawn : An NGS approach to mine SNP from the amplicons of Macrobrachium rosenbergii

Deepak Agarwal

Edit� par LAP LAMBERT Academic Publishing, 2019

ISBN 10 : 6200440050 ISBN 13 : 9786200440051

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Vendeur : AHA-BUCH GmbH, Einbeck, Allemagne

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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Amongst farmed crustaceans, the giant freshwater prawn Macrobrachium rosenbergii was until recently one of the major species with high commercial value. Lately, it has lost popularity due to diseases and differential growth of males on account of social hierarchy. The species can reacquire its former preferred status if it is genetically improved for growth and disease resistance. Selective breeding is greatly facilitated by marker-assisted selection (MAS). Single nucleotide polymorphisms (SNPs) offer the finest resolution possible for a marker map (a single nucleotide change), are co-dominant, bi-allelic and generally abundant in populations with a low mutation rate. DNA sequencing through NGS platforms is regarded as the most cost-effective approach. In this study, SNP mining was carried out in the transcribed sequences of M. rosenbergii available in GenBank, NCBI. Wild specimens were collected from different water bodies. Primers were designed to amplify coding (CDS) and sequenced the amplicons on GS-FLX 454 and Ion torrent platforms. 173 SNPs were detected across populations. This approach for mining SNPs in reported sequences has been used by us for the first time. N� de r�f. du vendeur 9786200440051

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