Degradation of LPVC by Laccase producing soil fungi: Degradation of Low Molecular Weight PolyvinylChloride (LPVC) by Laccase producing Soil Fungi, isolated from Plastic Dump - Couverture souple

Tirupathi, Sumathi; D.V.R, Sai Gopal

Couverture souple

ISBN 10 : 6200481938 ISBN 13 : 9786200481931

Editeur : LAP LAMBERT Academic Publishing, 2019

Synopsis

Among two strains were exhibited laccase activity DVRSGPD-1 and DVRSGPD-2 have exhibited laccase activity and hence we have used these two cultures for further studies. Based on morphological, molecular analysis it was confirmed that the cultures DVRSGPD-1 was closely related to the sequences of Cochliobolus hawaiiensis and DVRSGPD-2 to Aspergillus niger. The sequence database was deposited in NCBI GenBank and obtained an accession number KF805051 and KX055889. Cochliobolus hawaiiensis and Aspergillus niger were screened for optimization of the medium, pH, temperature, like carbon, nitrogen, inducers, agitation, CuSo4, and concentrations for production of higher laccase activity. Cochliobolus hawaiiensis was showed the best results than Aspergillus niger in all optimization parameters, so the Cochliobolus hawaiiensis was processed for further analysis.• The laccase enzyme produced by Cochliobolus hawaiiensis isolate was purified by ammonium sulfate method and the LPVC treated with Cochliobolus hawaiiensis and resulted metabolites were subjected to SEM, FTIR, NMR, GC - MS compared with control LPVC.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2019
• Langue: anglais
• ISBN 10: 6200481938
• ISBN 13: 9786200481931
• Reliure: Broché
• Nombre de pages: 288
• Coordonnées du fabricant: Buchpark GmbH; https://www.buchpark.de; ; Krügerweg 1; Trebbin; Brandenburg; 14959; Allemagne