Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus - Couverture souple

Name: Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Author: Shrivastava, Neeraj; Sharma, Bhaskar; Shrivastav, Arpita
ISBN: 9786200587886

Shrivastava, Neeraj ; Sharma, Bhaskar ; Shrivastav, Arpita

9786200587886: Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus

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ISBN 10 : 6200587884 ISBN 13 : 9786200587886

Editeur : LAP LAMBERT Academic Publishing, 2020

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The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF’ cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.

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�diteur: LAP LAMBERT Academic Publishing
Date d'�dition: 2020
Langue: anglais
ISBN 10: 6200587884
ISBN 13: 9786200587886
Reliure: Broch�
Nombre de pages: 64
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus

Neeraj Shrivastava

Edit� par LAP LAMBERT Academic Publishing Feb 2020, 2020

ISBN 10 : 6200587884 ISBN 13 : 9786200587886

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Vendeur : BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Allemagne

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low. 64 pp. Englisch. N� de r�f. du vendeur 9786200587886

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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus

Neeraj Shrivastava

Edit� par LAP LAMBERT Academic Publishing, 2020

ISBN 10 : 6200587884 ISBN 13 : 9786200587886

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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low. N� de r�f. du vendeur 9786200587886

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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus

Neeraj Shrivastava|Bhaskar Sharma|Arpita Shrivastav

Edit� par LAP LAMBERT Academic Publishing, 2020

ISBN 10 : 6200587884 ISBN 13 : 9786200587886

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Shrivastava NeerajDr Neeraj Shrivastava (Ph.D) is working as Assistant Professor in the Department of Veterinary Microbiology at N.D.V.S.U.,Jabalpur(M.P.)The present work is his MVSc thesis.Dr Shrivastava is a gold medalist in B.V.Sc. N� de r�f. du vendeur 385895895

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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus

Neeraj Shrivastava

Edit� par LAP LAMBERT Academic Publishing Feb 2020, 2020

ISBN 10 : 6200587884 ISBN 13 : 9786200587886

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Vendeur : buchversandmimpf2000, Emtmannsberg, BAYE, Allemagne

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.VDM Verlag, Dudweiler Landstra�e 99, 66123 Saarbr�cken 64 pp. Englisch. N� de r�f. du vendeur 9786200587886

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