Articles liés à Bacterial Gene Expression Technologies

Bacterial Gene Expression Technologies - Couverture souple

Zaky, Mahmoud; Abou-Selima, Merna

 
9786202004787: Bacterial Gene Expression Technologies
Couverture souple
ISBN 10 : 6202004789 ISBN 13 : 9786202004787
Editeur : LAP LAMBERT Academic Publishing, 2017

Synopsis

Random integration of the IVET vector into the pathogen chromosome is performed by insertion–duplication mutagenesis to create a pool of recombinant pathogens (this means that the gene in which the vector has inserted by homologous recombination is not disrupted). Recombinants can be selected using antibiotic resistance, as an additional marker is also on the integrated IVET construct. Pooled clones are then inoculated into the mouse. The two main types of IVET promoter trap strategies are (a) the complementation of auxotrophic mutation and (b) the expression of antibiotic resistance. Only those bacteria that contain the selective marker fused to a gene that is transcriptionally active in the host are able to survive. After a suitable infection period, bacteria that express the marker are isolated from lung, liver, spleen, and mesenteric tissue.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Random integration of the IVET vector into the pathogen chromosome is performed by insertion–duplication mutagenesis to create a pool of recombinant pathogens (this means that the gene in which the vector has inserted by homologous recombination is not disrupted). Recombinants can be selected using antibiotic resistance, as an additional marker is also on the integrated IVET construct. Pooled clones are then inoculated into the mouse. The two main types of IVET promoter trap strategies are (a) the complementation of auxotrophic mutation and (b) the expression of antibiotic resistance. Only those bacteria that contain the selective marker fused to a gene that is transcriptionally active in the host are able to survive. After a suitable infection period, bacteria that express the marker are isolated from lung, liver, spleen, and mesenteric tissue.

Biographie de l'auteur

Dr. Mahmoud M. M. Zaky, born in 23-09-1967, Cairo, Egypt. B.Sc in botany, Suez Canal University, Egypt. M.Sc in microbiology, joint Supervision, Suez Canal University and Portsmouth University, UK. Ph.D in molecular microbiology, joint supervision, Mansoura University, Egypt and Munich University, Germany.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2017
Langue
anglais
ISBN 10 
6202004789
ISBN 13 
9786202004787
Reliure
Broché
Nombre de pages
124
Coordonnées du fabricant
non disponible
Personne responsable
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