Molecular Cloning of Staphylococcus Aureus Alpha Hemolysin Toxin Gene - Couverture souple

Darwesh, Nada; Abdel-Fattah, Gamal; Hafez, Elsayed

Couverture souple

ISBN 10 : 6202197188 ISBN 13 : 9786202197182

Editeur : LAP LAMBERT Academic Publishing, 2017

Synopsis

Recombinant technology has crucial impact in therapy development. In microbial environment, Pathogenic bacteria such as Staphylococcus aureus produces alpha hemolysin protein. This protein is used as anticancer protein. alpha hemolysin gene was isolated from pathogenic S. aureus that was isolated from blood samples of patients (chrildren). This recombinant hia gene was produced alpha hemolysin protein by using competent E. coli as a host. The recombinant α- toxin protein was used as anticancer agent.In this study that high concentration of α-toxin (500 μl/ ml) was effected on the cell viability of four cells line ( HepG-2, HCT-116, MCF-7, A-549). The cell viability of cell lines were represented 31.69% in HepG-2, 36.27% in HCT-116, 78.95% in MCF-7, 67.28% in A-549. From our results, Alpha hemolysin protein had high inhibitory activity against HepG-2 and HCT-116, and had weak inhibitory activity against MCF-7 and A-549. Inconclusion,The production of recombinant α- toxin was very simple process, low cost, high efficiency, non-toxic, high yield and no side effect.

A propos du livre

À propos de l'auteur

The AuthorNada Darwesh obtained Bachelor of Science in Botany / Chemistry (2005), Master Degree in Microbiology (2012) and Doctor Degree in Microbiology (2017) from Faculty of Science, Mansoura University, Egypt.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2017
• Langue: anglais
• ISBN 10: 6202197188
• ISBN 13: 9786202197182
• Reliure: Broché
• Nombre de pages: 128
• Coordonnées du fabricant: non disponible
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