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Construction of plasmid-based expression vector for Bacillus subtilis: In cytoplasm, on the surface and as secretion into culture medium - Couverture souple

Nguyen, Hoang D.

 
9786202300728: Construction of plasmid-based expression vector for Bacillus subtilis: In cytoplasm, on the surface and as secretion into culture medium
Couverture souple
ISBN 10 : 6202300728 ISBN 13 : 9786202300728
Editeur : Scholars' Press, 2017

Synopsis

Plasmids are useful tools to study gene expression and their function. However, most of the available plasmids for Bacillus subtilis suffer from structural instability because of their rolling-circle replication mechanism. In this work, stable plasmids have been constructed allowing expression of recombinant proteins in the cytoplasm and their secretion into the culture supernatant. The author has also established an experimental system to immobilize proteins on the cell wall of B. subtilis. The sorting of surface proteins to the cell wall in B. subtilis has been investigated aiming to anchor recombinant protein on the surface.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Plasmids are useful tools to study gene expression and their function. However, most of the available plasmids for Bacillus subtilis suffer from structural instability because of their rolling-circle replication mechanism. In this work, stable plasmids have been constructed allowing expression of recombinant proteins in the cytoplasm and their secretion into the culture supernatant. The author has also established an experimental system to immobilize proteins on the cell wall of B. subtilis. The sorting of surface proteins to the cell wall in B. subtilis has been investigated aiming to anchor recombinant protein on the surface.

Biographie de l'auteur

Hoang D. Nguyen obtained his Ph.D. certificate at the University of Bayreuth, Germany. He is now an associate professor of Microbiology at the University of Science, Vietnam National University-Hochiminh City. He has a keen interest in the development of expression systems for Bacillus subtilis and production of recombinant proteins.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Scholars' Press
Date d'édition
2017
Langue
anglais
ISBN 10 
6202300728
ISBN 13 
9786202300728
Reliure
Broché
Nombre de pages
136
Coordonnées du fabricant
non disponible
Personne responsable
non disponible