Articles liés à Class I HLA in Normal Human Tissues: Laboratory Data.:...

Class I HLA in Normal Human Tissues: Laboratory Data.: Quantification by RT-PCR - Couverture souple

García-Ruano, Ana Belén; Martín-Alcolea, María Amparo; García-Chileme, Soledad

 
9786203718850: Class I HLA in Normal Human Tissues: Laboratory Data.: Quantification by RT-PCR
Couverture souple
ISBN 10 : 6203718858 ISBN 13 : 9786203718850
Editeur : Our Knowledge Publishing, 2021

Synopsis

To know if the transcription level of a certain gene (HLA-A, HLA-B or HLA-C) is altered in tumour cells, we should first know what the transcription level is in the normal cells from which these tumours derive.Therefore, in our study we will try to quantitatively measure the locus-specific gene expression of HLA-ABC in normal tissues. In order to perform this measurement we have designed primers that specifically amplify these locus by real-time PCR. The normal tissues chosen were peripheral blood leukocytes and colon and larynx mucosa. In order to select the appropriate cells, thus avoiding interferences, laser microdissection has been performed in both colon and larynx mucosa. Throughout this work we will provide evidence of the specificity of the technique as well as the patterns of gene expression found in the different tissues studied.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Éditeur
Our Knowledge Publishing
Date d'édition
2021
Langue
anglais
ISBN 10 
6203718858
ISBN 13 
9786203718850
Reliure
Broché
Nombre de pages
52
Coordonnées du fabricant
non disponible
Personne responsable
non disponible